Heterologous protein expression in filamentous fungi

K. M Helena Nevalainen*, Valentino S J Te'o, Peter L. Bergquist

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

237 Citations (Scopus)


Filamentous fungi are commonly used in the fermentation industry for the large-scale production of proteins - mainly industrial enzymes. Recent advances in fungal genomics and related experimental technologies such as gene arrays and proteomics are rapidly changing the approaches to the development and use of filamentous fungi as hosts for the production of both homologous and heterologous gene products. The emphasis is moving towards sourcing new genes of interest through database mining and unravelling the circuits related to fungal gene regulation, applying, for example, transcriptomics. However, although heterologous fungal proteins are efficiently expressed, expression of gene products from other organisms is subject to several bottlenecks that reduce yield. Current approaches emphasize the study of pathways involved in protein modification and degradation in general rather than gene-by-gene approaches.

Original languageEnglish
Pages (from-to)468-474
Number of pages7
JournalTrends in Biotechnology
Issue number9
Publication statusPublished - Sept 2005


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