Heterophilic antibodies

A problem for all immunoassays

L. M. Boscato, M. C. Stuart

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

We verified that antibody-binding substances in serum that interfere in two-site immunoassays involving murine antibodies are heterophilic antibodies. Incubations of serum containing heterophilic antibodies and a murine monoclonal antibody to human choriogonadotropin (hCG) leads to formation of a series of soluble immune complexes. We investigated the recognition of hCG by reagent antibody in the presence of heterophilic antibodies and found this recognition to be diminished. Consequently, about 30% of serum samples containing heterophilic antibodies falsely appear to contain increased concentrations of hCG. The effect on analyte recognition probably results from steric inhibition of hCG binding to complexed antibody. Heterophilic antibodies detected with a murine antibody also bound immunoglobulin from several other species but did not bind all of those tested.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalClinical Chemistry
Volume34
Issue number1
Publication statusPublished - 1988

Fingerprint Dive into the research topics of 'Heterophilic antibodies: A problem for all immunoassays'. Together they form a unique fingerprint.

Cite this