We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.
|Number of pages||4|
|Publication status||Published - 1989|