High expression in mammalian cells without amplification

Jeffrey S. Friedman, Claire L. Cofer, Cheryl L. Anderson, Jake A. Kushner, Peter P. Gray, George E. Chapman, Margaret C. Stuart, Leslie Lazarus, John Shine, Peter J. Kushner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.

Original languageEnglish
Pages (from-to)359-362
Number of pages4
Issue number4
Publication statusPublished - 1989


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