High expression in mammalian cells without amplification

Jeffrey S. Friedman; Claire L. Cofer; Cheryl L. Anderson; Jake A. Kushner; Peter P. Gray; George E. Chapman; Margaret C. Stuart; Leslie Lazarus; John Shine; Peter J. Kushner

doi:10.1038/nbt0489-359

High expression in mammalian cells without amplification

Jeffrey S. Friedman, Claire L. Cofer, Cheryl L. Anderson, Jake A. Kushner, Peter P. Gray, George E. Chapman, Margaret C. Stuart, Leslie Lazarus, John Shine, Peter J. Kushner^*

^*Corresponding author for this work

Deputy Vice-Chancellor (Research) - Office

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.

Original language	English
Pages (from-to)	359-362
Number of pages	4
Journal	Bio/Technology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1038/nbt0489-359
Publication status	Published - 1989

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title = "High expression in mammalian cells without amplification",

abstract = "We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.",

author = "Friedman, \{Jeffrey S.\} and Cofer, \{Claire L.\} and Anderson, \{Cheryl L.\} and Kushner, \{Jake A.\} and Gray, \{Peter P.\} and Chapman, \{George E.\} and Stuart, \{Margaret C.\} and Leslie Lazarus and John Shine and Kushner, \{Peter J.\}",

year = "1989",

doi = "10.1038/nbt0489-359",

language = "English",

volume = "7",

pages = "359--362",

journal = "Bio/Technology",

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T1 - High expression in mammalian cells without amplification

AU - Friedman, Jeffrey S.

AU - Cofer, Claire L.

AU - Anderson, Cheryl L.

AU - Kushner, Jake A.

AU - Gray, Peter P.

AU - Chapman, George E.

AU - Stuart, Margaret C.

AU - Lazarus, Leslie

AU - Shine, John

AU - Kushner, Peter J.

PY - 1989

Y1 - 1989

N2 - We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.

AB - We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metalloth- ionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.

UR - https://www.scopus.com/pages/publications/84970062371

U2 - 10.1038/nbt0489-359

DO - 10.1038/nbt0489-359

M3 - Article

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SN - 0733-222X

VL - 7

SP - 359

EP - 362

JO - Bio/Technology

JF - Bio/Technology

IS - 4

ER -

High expression in mammalian cells without amplification

Abstract

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