High-glycemic index carbohydrate increases nuclear factor-κB activation in mononuclear cells of young, lean healthy subjects1-3

Scott Dickinson, Dale P. Hancock, Peter Petocz, Antonio Ceriello, Jennie Brand-Miller*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    148 Citations (Scopus)


    Background: High-glycemic index diets have been linked to greater risk of cardiovascular disease and type 2 diabetes. Postprandial glycemia within the normal range may promote oxidative stress and inflammatory processes underlying the development of disease. Objective: We explored acute differences in the activation of the inflammatory marker nuclear factor-κB after consumption of 2 carbohydrate meals matched for macronutrient and micronutrient composition but differing in glycemic index. Design: After an overnight fast, 10 young, lean healthy subjects were fed in random order 3 meals providing 50 g of available carbohydrate as glucose, white bread, or pasta. Venous blood samples were collected at 0, 1, 2, and 3 h, and nuclear proteins were extracted from mononuclear cells. Changes in nuclear factor-κB-p65 proteins were detected by Western blotting. Acute changes in other markers of oxidative stress (nitrotyrosine and soluble intercellular adhesion molecule-1) were also assessed. Results: The maximum increase in nuclear factor-κBactivation was similar after the bread meal [mean (±SEM) area under the curve: 69 ± 16% optical density · h] and the glucose challenge (75 ± 9% optical density · h), but was 3 times higher than after the pasta meal (23 ± 5% optical density · h) (P < 0.05). Similarly, changes in nitrotyrosine, but not soluble intercellular adhesion molecule-1, were higher after glucose and bread than after pasta (P=0.01 at 2 h). Conclusions: The findings suggest that high-normal physiologic increases in blood glucose after meals aggravate inflammatory processes in lean, young adults. This mechanism may help to explain relations between carbohydrates, glycemic index, and the risk of chronic disease.

    Original languageEnglish
    Pages (from-to)1188-1193
    Number of pages6
    JournalAmerican Journal of Clinical Nutrition
    Issue number5
    Publication statusPublished - 1 May 2008


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