High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast

Hangrui Liu; Kai Peng; James A. Piper; Ming Li

High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast

Hangrui Liu, Kai Peng, James A. Piper, Ming Li^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract

Abstract

We present a high-throughput double emulsion (DE) droplet-based method for the screening and sorting of yeast (Saccharomyces cerevisiae) factories that can produce the industrial enzyme, β-Glucosidase (BGL). The formed DEs allows long-term culture (i.e., 4.5 hours) culture of encapsulated cells without any leakage or coalesce, and flow cytometric quantification and sorting. To the best of our knowledge, it is the first report on the screening and sorting of BGL enzyme activity in single yeast cells using droplet-based microfluidic technique.

Original language	English
Title of host publication	MicroTAS 2021
Subtitle of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Publication	Palm Springs, CA
Publisher	Chemical and Biological Microsystems Society
Pages	987-988
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

Keywords

double emulsions
enzyme activity
high-throughput screening
sorting
yeast

Cite this

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title = "High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast",

abstract = "We present a high-throughput double emulsion (DE) droplet-based method for the screening and sorting of yeast (Saccharomyces cerevisiae) factories that can produce the industrial enzyme, β-Glucosidase (BGL). The formed DEs allows long-term culture (i.e., 4.5 hours) culture of encapsulated cells without any leakage or coalesce, and flow cytometric quantification and sorting. To the best of our knowledge, it is the first report on the screening and sorting of BGL enzyme activity in single yeast cells using droplet-based microfluidic technique.",

keywords = "double emulsions, enzyme activity, high-throughput screening, sorting, yeast",

author = "Hangrui Liu and Kai Peng and Piper, {James A.} and Ming Li",

year = "2021",

language = "English",

pages = "987--988",

booktitle = "MicroTAS 2021",

publisher = "Chemical and Biological Microsystems Society",

note = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

}

Liu, H, Peng, K, Piper, JA & Li, M 2021, High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast. in MicroTAS 2021: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, Palm Springs, CA, pp. 987-988, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast. / Liu, Hangrui; Peng, Kai; Piper, James A. et al.
MicroTAS 2021: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Palm Springs, CA: Chemical and Biological Microsystems Society, 2021. p. 987-988.

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract

TY - CHAP

T1 - High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast

AU - Liu, Hangrui

AU - Peng, Kai

AU - Piper, James A.

AU - Li, Ming

PY - 2021

Y1 - 2021

N2 - We present a high-throughput double emulsion (DE) droplet-based method for the screening and sorting of yeast (Saccharomyces cerevisiae) factories that can produce the industrial enzyme, β-Glucosidase (BGL). The formed DEs allows long-term culture (i.e., 4.5 hours) culture of encapsulated cells without any leakage or coalesce, and flow cytometric quantification and sorting. To the best of our knowledge, it is the first report on the screening and sorting of BGL enzyme activity in single yeast cells using droplet-based microfluidic technique.

AB - We present a high-throughput double emulsion (DE) droplet-based method for the screening and sorting of yeast (Saccharomyces cerevisiae) factories that can produce the industrial enzyme, β-Glucosidase (BGL). The formed DEs allows long-term culture (i.e., 4.5 hours) culture of encapsulated cells without any leakage or coalesce, and flow cytometric quantification and sorting. To the best of our knowledge, it is the first report on the screening and sorting of BGL enzyme activity in single yeast cells using droplet-based microfluidic technique.

KW - double emulsions

KW - enzyme activity

KW - high-throughput screening

KW - sorting

KW - yeast

UR - http://www.scopus.com/inward/record.url?scp=85136951376&partnerID=8YFLogxK

UR - http://purl.org/au-research/grants/arc/DP200102269

UR - http://purl.org/au-research/grants/arc/CE140100003

M3 - Conference abstract

AN - SCOPUS:85136951376

SP - 987

EP - 988

BT - MicroTAS 2021

PB - Chemical and Biological Microsystems Society

CY - Palm Springs, CA

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast

Abstract

Conference

Keywords

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High-throughput microfluidics for the screening and sorting of superior cellulase activity in yeast

Abstract

Conference

Keywords

Other files and links

Fingerprint

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Cell-Sort MultiTool: a Novel Platform for Single-cell Bacteria Analysis

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