High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Siu F. Lee*, Vanessa L. White, Andrew R. Weeks, Ary A. Hoffmann, Nancy M. Endersby

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

Original languageEnglish
Pages (from-to)4740-4743
Number of pages4
JournalApplied and Environmental Microbiology
Volume78
Issue number13
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

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