High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Siu F. Lee; Vanessa L. White; Andrew R. Weeks; Ary A. Hoffmann; Nancy M. Endersby

doi:10.1128/AEM.00069-12

High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Siu F. Lee^*, Vanessa L. White, Andrew R. Weeks, Ary A. Hoffmann, Nancy M. Endersby

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

Original language	English
Pages (from-to)	4740-4743
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	13
DOIs	https://doi.org/10.1128/AEM.00069-12
Publication status	Published - Jul 2012
Externally published	Yes

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10.1128/AEM.00069-12

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title = "High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans",

abstract = "We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.",

author = "Lee, {Siu F.} and White, {Vanessa L.} and Weeks, {Andrew R.} and Hoffmann, {Ary A.} and Endersby, {Nancy M.}",

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AU - White, Vanessa L.

AU - Weeks, Andrew R.

AU - Hoffmann, Ary A.

AU - Endersby, Nancy M.

PY - 2012/7

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AB - We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Abstract

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