High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes

Femia G. Hopwood; Janice L. Duff; Cameron J. Hill; Paul E. Smith; Parag S. Gandhi; Wendy Holstein; Nicole L. Wilson; Nicolle H. Packer; Patrick W. Cooley; David B. Wallace; Andrew A. Gooley

High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes

Femia G. Hopwood^*, Janice L. Duff, Cameron J. Hill, Paul E. Smith, Parag S. Gandhi, Wendy Holstein, Nicole L. Wilson, Nicolle H. Packer, Patrick W. Cooley, David B. Wallace, Andrew A. Gooley

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

A flexible automated approach to peptide mass fingerprinting (PMF) was described using the prototype chemical printer (CHiP). Human plasma platelets were separated with 2D electrophoresis and electroblotted onto PVDF membrane and adhered to a MALDI target. The plasma spots labelled in the 2D blot were processed on the CHiP and analysed by MALDI-TOF)MS directly on the membrane surface. The CHiP technology provided a strategy for archiving valuable samples and probing candidate proteins with specific chemistries.

Original language	English
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Publication	Chicago
Publisher	American Society for Mass Spectrometry
Pages	169-170
Number of pages	2
Publication status	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

Hopwood, F. G., Duff, J. L., Hill, C. J., Smith, P. E., Gandhi, P. S., Holstein, W., Wilson, N. L., Packer, N. H., Cooley, P. W., Wallace, D. B., & Gooley, A. A. (2002). High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 169-170). American Society for Mass Spectrometry.

@inproceedings{52658809902e44c8a46beb5f46bb0fba,

title = "High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes",

abstract = "A flexible automated approach to peptide mass fingerprinting (PMF) was described using the prototype chemical printer (CHiP). Human plasma platelets were separated with 2D electrophoresis and electroblotted onto PVDF membrane and adhered to a MALDI target. The plasma spots labelled in the 2D blot were processed on the CHiP and analysed by MALDI-TOF)MS directly on the membrane surface. The CHiP technology provided a strategy for archiving valuable samples and probing candidate proteins with specific chemistries.",

author = "Hopwood, {Femia G.} and Duff, {Janice L.} and Hill, {Cameron J.} and Smith, {Paul E.} and Gandhi, {Parag S.} and Wendy Holstein and Wilson, {Nicole L.} and Packer, {Nicolle H.} and Cooley, {Patrick W.} and Wallace, {David B.} and Gooley, {Andrew A.}",

year = "2002",

language = "English",

pages = "169--170",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

publisher = "American Society for Mass Spectrometry",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Hopwood, FG, Duff, JL, Hill, CJ, Smith, PE, Gandhi, PS, Holstein, W, Wilson, NL, Packer, NH, Cooley, PW, Wallace, DB & Gooley, AA 2002, High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. American Society for Mass Spectrometry, Chicago, pp. 169-170, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 2/06/02.

High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes. / Hopwood, Femia G.; Duff, Janice L.; Hill, Cameron J. et al.

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. Chicago : American Society for Mass Spectrometry, 2002. p. 169-170.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes

AU - Hopwood, Femia G.

AU - Duff, Janice L.

AU - Hill, Cameron J.

AU - Smith, Paul E.

AU - Gandhi, Parag S.

AU - Holstein, Wendy

AU - Wilson, Nicole L.

AU - Packer, Nicolle H.

AU - Cooley, Patrick W.

AU - Wallace, David B.

AU - Gooley, Andrew A.

PY - 2002

Y1 - 2002

N2 - A flexible automated approach to peptide mass fingerprinting (PMF) was described using the prototype chemical printer (CHiP). Human plasma platelets were separated with 2D electrophoresis and electroblotted onto PVDF membrane and adhered to a MALDI target. The plasma spots labelled in the 2D blot were processed on the CHiP and analysed by MALDI-TOF)MS directly on the membrane surface. The CHiP technology provided a strategy for archiving valuable samples and probing candidate proteins with specific chemistries.

AB - A flexible automated approach to peptide mass fingerprinting (PMF) was described using the prototype chemical printer (CHiP). Human plasma platelets were separated with 2D electrophoresis and electroblotted onto PVDF membrane and adhered to a MALDI target. The plasma spots labelled in the 2D blot were processed on the CHiP and analysed by MALDI-TOF)MS directly on the membrane surface. The CHiP technology provided a strategy for archiving valuable samples and probing candidate proteins with specific chemistries.

UR - http://www.scopus.com/inward/record.url?scp=2442715481&partnerID=8YFLogxK

M3 - Conference proceeding contribution

AN - SCOPUS:2442715481

SP - 169

EP - 170

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

PB - American Society for Mass Spectrometry

CY - Chicago

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes

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