High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes

Femia G. Hopwood*, Janice L. Duff, Cameron J. Hill, Paul E. Smith, Parag S. Gandhi, Wendy Holstein, Nicole L. Wilson, Nicolle H. Packer, Patrick W. Cooley, David B. Wallace, Andrew A. Gooley

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

A flexible automated approach to peptide mass fingerprinting (PMF) was described using the prototype chemical printer (CHiP). Human plasma platelets were separated with 2D electrophoresis and electroblotted onto PVDF membrane and adhered to a MALDI target. The plasma spots labelled in the 2D blot were processed on the CHiP and analysed by MALDI-TOF)MS directly on the membrane surface. The CHiP technology provided a strategy for archiving valuable samples and probing candidate proteins with specific chemistries.

Original languageEnglish
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Place of PublicationChicago
PublisherAmerican Society for Mass Spectrometry
Pages169-170
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period2/06/026/06/02

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    Hopwood, F. G., Duff, J. L., Hill, C. J., Smith, P. E., Gandhi, P. S., Holstein, W., ... Gooley, A. A. (2002). High throughput peptide mass fingerprinting and post translational modification for proteomics utilising piezoelectric microdispensing and PVDF membranes. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 169-170). Chicago: American Society for Mass Spectrometry.