Highly accurate deterministic lateral displacement device and its application to purification of fungal spores

David W. Inglis; Nick Herman; Graham Vesey

doi:10.1063/1.3430553

Highly accurate deterministic lateral displacement device and its application to purification of fungal spores

David W. Inglis^*, Nick Herman, Graham Vesey

^*Corresponding author for this work

MQ Photonics Research Centre

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

9 Downloads (Pure)

Abstract

We have designed, built, and evaluated a microfluidic device that uses deterministic lateral displacement for size-based separation. The device achieves almost 100% purity and recovery in continuously sorting two, four, and six micrometer microspheres. We have applied this highly efficient device to the purification of fungal (Aspergillus) spores that are spherical (∼4 μm diameter) with a narrow size distribution. Such separation directly from culture using unfiltered A. niger suspensions is difficult due to a high level of debris. The device produces a two to three increase in the ratio of spores to debris as measured by light scatter in a flow cytometer. The procedure is feasible at densities up to 4.4× ¹⁰⁶ spores/ml. This is one of the first studies to apply microfluidic techniques to spore separations and has demonstrated that a passive separation system could significantly reduce the amount of debris in a suspension of fungal spores with virtually no loss of spore material.

Original language	English
Article number	010002BMF
Pages (from-to)	024109-1-024109-8
Number of pages	8
Journal	Biomicrofluidics
Volume	4
Issue number	2
DOIs	https://doi.org/10.1063/1.3430553
Publication status	Published - Jun 2010

Bibliographical note

Copyright (2010) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics, 4:2, 024109 (2010) and may be found at http://link.aip.org/link/doi/10.1063/1.3430553.

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title = "Highly accurate deterministic lateral displacement device and its application to purification of fungal spores",

abstract = "We have designed, built, and evaluated a microfluidic device that uses deterministic lateral displacement for size-based separation. The device achieves almost 100% purity and recovery in continuously sorting two, four, and six micrometer microspheres. We have applied this highly efficient device to the purification of fungal (Aspergillus) spores that are spherical (∼4 μm diameter) with a narrow size distribution. Such separation directly from culture using unfiltered A. niger suspensions is difficult due to a high level of debris. The device produces a two to three increase in the ratio of spores to debris as measured by light scatter in a flow cytometer. The procedure is feasible at densities up to 4.4× 106 spores/ml. This is one of the first studies to apply microfluidic techniques to spore separations and has demonstrated that a passive separation system could significantly reduce the amount of debris in a suspension of fungal spores with virtually no loss of spore material.",

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AB - We have designed, built, and evaluated a microfluidic device that uses deterministic lateral displacement for size-based separation. The device achieves almost 100% purity and recovery in continuously sorting two, four, and six micrometer microspheres. We have applied this highly efficient device to the purification of fungal (Aspergillus) spores that are spherical (∼4 μm diameter) with a narrow size distribution. Such separation directly from culture using unfiltered A. niger suspensions is difficult due to a high level of debris. The device produces a two to three increase in the ratio of spores to debris as measured by light scatter in a flow cytometer. The procedure is feasible at densities up to 4.4× 106 spores/ml. This is one of the first studies to apply microfluidic techniques to spore separations and has demonstrated that a passive separation system could significantly reduce the amount of debris in a suspension of fungal spores with virtually no loss of spore material.

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Highly accurate deterministic lateral displacement device and its application to purification of fungal spores

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