Highly sensitive and selective phosphorescent chemosensors for hypochlorous acid based on ruthenium(II) complexes

Run Zhang, Bo Song, Zhichao Dai, Zhiqiang Ye*, Yunna Xiao, Yan Liu, Jingli Yuan

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    Microbicidal activity of hypochlorous acid (HOCl) plays pivotal roles in many biological processes, and suitable methods for the in vivo detection of HOCl are highly desirable. Herein we report the development of two novel ruthenium(II) complex-based phosphorescent chemosensors for HOCl, [Ru(bpy)2(DNCA-bpy)](PF6)2 (bpy: 2,2'-bipyridine; DNCA-bpy: 4-N-(2,4-dinitrophenyl)carboxamide-4'-methyl-2,2'-bipyridine) and [Ru(bpy)2(DNCH-bpy)](PF6)2 (DNCH-bpy: 4-N'-(2,4-dinitrophenyl)carbohydrazide-4'-methyl-2,2'-bipyridine). The two sensors are almost non-luminescent due to the intramolecular photoinduced electron transfer (PET) process from the Ru(II)-bpy moiety to dinitrophenyl group, but specific reaction of the sensors with HOCl in aqueous media can afford a highly luminescent derivative, [Ru(bpy)2(COOH-bpy)](PF6)2 (COOH-bpy: 4-carboxylic acid-4'-methyl-2,2'-bipyridine), accompanied by 190-fold and 1100-fold turn-on luminescence signal enhancement for [Ru(bpy)2(DNCA-bpy)](PF6)2 and [Ru(bpy)2(DNCH-bpy)](PF6)2, respectively. By taking advantage of high specificity and sensitivity of the chemosensor and excellent photophysical property of Ru(II) complex, [Ru(bpy)2(DNCH-bpy)](PF6)2 was applied for visualizing the endogenous HOCl generation in living macrophage cells during the processes of stimulation and phagocytosis via the red-emitting luminescence.

    Original languageEnglish
    Pages (from-to)1-7
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume50
    DOIs
    Publication statusPublished - 15 Dec 2013

    Keywords

    • Hypochlorous acid
    • Luminescence imaging
    • Phosphorescent chemosensor
    • Ruthenium(II) complex

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