TY - JOUR
T1 - Highly sensitive and selective phosphorescent chemosensors for hypochlorous acid based on ruthenium(II) complexes
AU - Zhang, Run
AU - Song, Bo
AU - Dai, Zhichao
AU - Ye, Zhiqiang
AU - Xiao, Yunna
AU - Liu, Yan
AU - Yuan, Jingli
PY - 2013/12/15
Y1 - 2013/12/15
N2 - Microbicidal activity of hypochlorous acid (HOCl) plays pivotal roles in many biological processes, and suitable methods for the in vivo detection of HOCl are highly desirable. Herein we report the development of two novel ruthenium(II) complex-based phosphorescent chemosensors for HOCl, [Ru(bpy)2(DNCA-bpy)](PF6)2 (bpy: 2,2'-bipyridine; DNCA-bpy: 4-N-(2,4-dinitrophenyl)carboxamide-4'-methyl-2,2'-bipyridine) and [Ru(bpy)2(DNCH-bpy)](PF6)2 (DNCH-bpy: 4-N'-(2,4-dinitrophenyl)carbohydrazide-4'-methyl-2,2'-bipyridine). The two sensors are almost non-luminescent due to the intramolecular photoinduced electron transfer (PET) process from the Ru(II)-bpy moiety to dinitrophenyl group, but specific reaction of the sensors with HOCl in aqueous media can afford a highly luminescent derivative, [Ru(bpy)2(COOH-bpy)](PF6)2 (COOH-bpy: 4-carboxylic acid-4'-methyl-2,2'-bipyridine), accompanied by 190-fold and 1100-fold turn-on luminescence signal enhancement for [Ru(bpy)2(DNCA-bpy)](PF6)2 and [Ru(bpy)2(DNCH-bpy)](PF6)2, respectively. By taking advantage of high specificity and sensitivity of the chemosensor and excellent photophysical property of Ru(II) complex, [Ru(bpy)2(DNCH-bpy)](PF6)2 was applied for visualizing the endogenous HOCl generation in living macrophage cells during the processes of stimulation and phagocytosis via the red-emitting luminescence.
AB - Microbicidal activity of hypochlorous acid (HOCl) plays pivotal roles in many biological processes, and suitable methods for the in vivo detection of HOCl are highly desirable. Herein we report the development of two novel ruthenium(II) complex-based phosphorescent chemosensors for HOCl, [Ru(bpy)2(DNCA-bpy)](PF6)2 (bpy: 2,2'-bipyridine; DNCA-bpy: 4-N-(2,4-dinitrophenyl)carboxamide-4'-methyl-2,2'-bipyridine) and [Ru(bpy)2(DNCH-bpy)](PF6)2 (DNCH-bpy: 4-N'-(2,4-dinitrophenyl)carbohydrazide-4'-methyl-2,2'-bipyridine). The two sensors are almost non-luminescent due to the intramolecular photoinduced electron transfer (PET) process from the Ru(II)-bpy moiety to dinitrophenyl group, but specific reaction of the sensors with HOCl in aqueous media can afford a highly luminescent derivative, [Ru(bpy)2(COOH-bpy)](PF6)2 (COOH-bpy: 4-carboxylic acid-4'-methyl-2,2'-bipyridine), accompanied by 190-fold and 1100-fold turn-on luminescence signal enhancement for [Ru(bpy)2(DNCA-bpy)](PF6)2 and [Ru(bpy)2(DNCH-bpy)](PF6)2, respectively. By taking advantage of high specificity and sensitivity of the chemosensor and excellent photophysical property of Ru(II) complex, [Ru(bpy)2(DNCH-bpy)](PF6)2 was applied for visualizing the endogenous HOCl generation in living macrophage cells during the processes of stimulation and phagocytosis via the red-emitting luminescence.
KW - Hypochlorous acid
KW - Luminescence imaging
KW - Phosphorescent chemosensor
KW - Ruthenium(II) complex
UR - http://www.scopus.com/inward/record.url?scp=84880015128&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2013.06.005
DO - 10.1016/j.bios.2013.06.005
M3 - Article
C2 - 23827370
AN - SCOPUS:84880015128
SN - 0956-5663
VL - 50
SP - 1
EP - 7
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -