Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Yuling Wang; Eugene J H Wee; Matt Trau

doi:10.1039/c5cc03921e

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Yuling Wang^*, Eugene J H Wee, Matt Trau

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

Original language	English
Pages (from-to)	10953-10956
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	54
DOIs	https://doi.org/10.1039/c5cc03921e
Publication status	Published - 11 Jul 2015
Externally published	Yes

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title = "Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction",

abstract = "Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.",

author = "Yuling Wang and Wee, {Eugene J H} and Matt Trau",

year = "2015",

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doi = "10.1039/c5cc03921e",

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AU - Wang, Yuling

AU - Wee, Eugene J H

AU - Trau, Matt

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N2 - Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

AB - Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

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U2 - 10.1039/c5cc03921e

DO - 10.1039/c5cc03921e

M3 - Article

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AN - SCOPUS:84934935122

VL - 51

SP - 10953

EP - 10956

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 54

ER -

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Abstract

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