Abstract
Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.
Original language | English |
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Pages (from-to) | 10953-10956 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 51 |
Issue number | 54 |
DOIs | |
Publication status | Published - 11 Jul 2015 |
Externally published | Yes |