Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Yuling Wang; Eugene J H Wee; Matt Trau

doi:10.1039/c5cc03921e

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Yuling Wang^*, Eugene J H Wee, Matt Trau

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

Original language	English
Pages (from-to)	10953-10956
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	54
DOIs	https://doi.org/10.1039/c5cc03921e
Publication status	Published - 11 Jul 2015
Externally published	Yes

Access to Document

10.1039/c5cc03921e

Cite this

@article{72b061e3032f4526b6b4d983f0e490f8,

title = "Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction",

abstract = "Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.",

author = "Yuling Wang and Wee, {Eugene J H} and Matt Trau",

year = "2015",

month = jul,

day = "11",

doi = "10.1039/c5cc03921e",

language = "English",

volume = "51",

pages = "10953--10956",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "54",

}

TY - JOUR

T1 - Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

AU - Wang, Yuling

AU - Wee, Eugene J H

AU - Trau, Matt

PY - 2015/7/11

Y1 - 2015/7/11

N2 - Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

AB - Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

UR - http://www.scopus.com/inward/record.url?scp=84934935122&partnerID=8YFLogxK

U2 - 10.1039/c5cc03921e

DO - 10.1039/c5cc03921e

M3 - Article

C2 - 26063626

AN - SCOPUS:84934935122

SN - 1359-7345

VL - 51

SP - 10953

EP - 10956

JO - Chemical Communications

JF - Chemical Communications

IS - 54

ER -

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Abstract

Access to Document

Other files and links

Fingerprint

Cite this