Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

Yuling Wang*, Eugene J H Wee, Matt Trau

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method.

Original languageEnglish
Pages (from-to)10953-10956
Number of pages4
JournalChemical Communications
Volume51
Issue number54
DOIs
Publication statusPublished - 11 Jul 2015
Externally publishedYes

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