Highly variable immune-response proteins (185/333) from the sea urchin, Strongylocentrotus purpuratus: Proteomic analysis identifies diversity within and between individuals

Nolwenn M. Dheilly, Sham V. Nair, L. Courtney Smith, David A. Raftos

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

185/333 genes and transcripts from the purple sea urchin, Strongylocentrotus purpuratus, predict high levels of amino acid diversity within the encoded proteins. Based on their expression patterns, 185/333 proteins appear to be involved in immune responses. In the present study, one- and two-dimensional Western blots show that 185/333 proteins exhibit high levels of molecular diversity within and between individual sea urchins. The molecular masses of 185/333-positive bands or spots range from 30 to 250 kDa with a broad array of isoelectric points. The observed molecular masses are higher than those predicted from mRNAs, suggesting that 185/333 proteins form strong associations with other molecules or with each other. Some sea urchins expressed >200 distinct 185/333 proteins, and each animal had a unique suite of the proteins that differed from all other individuals. When sea urchins were challenged in vivo with pathogen-associated molecular patterns (PAMPs; bacterial LPS and peptidoglycan), the expression of 185/333 proteins increased. More importantly, different suites of 185/333 proteins were expressed in response to different PAMPs. This suggests that the expression of 185/333 proteins can be tailored toward different PAMPs in a form of pathogenspecific immune response.

Original languageEnglish
Pages (from-to)2203-2212
Number of pages10
JournalJournal of Immunology
Volume182
Issue number4
DOIs
Publication statusPublished - 15 Feb 2009

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