Host cell tropism of equine herpesviruses

Glycoprotein D of EHV-1 enables EHV-4 to infect a non-permissive cell line

J. M. Whalley*, K. M. Ruitenberg, K. Sullivan, L. Seshadri, K. Hansen, D. Birch, J. R. Gilkerson, J. E. Wellington

*Corresponding author for this work

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Abstract

Equine herpesviruses 1 and 4 (EHV-1 and EHV-4) cause equine respiratory disease worldwide. However, only EHV-1 is a cause of abortion and neurological disease, despite the two viruses having all 76 genes in common. In addition EHV-1 has a broader host range in cell culture than EHV-4, as exemplified by the rabbit kidney (RK) cell line that is permissive for EHV-1, but not for EHV-4. Here we describe that when EHV-4 produced in equine cells was inoculated onto RK cells expressing glycoprotein D of EHV-1 (RKgD1), infection developed as clusters of rounded cells, and this infectivity could be passaged in RKgD1 cells. The progeny virus could also infect single RK cells, consistent with EHV-4 acquiring EHV1∈gD from the complementing cell line. No such infection was observed for EHV-4 in RK cells expressing EHV-1 glycoprotein C. The results are consistent with gD homologues being major determinants of host cell tropism and raise the possibility that gD may be a factor in the differential pathogenicity of EHV-1 and EHV-4.

Original languageEnglish
Pages (from-to)717-725
Number of pages9
JournalArchives of Virology
Volume152
Issue number4
DOIs
Publication statusPublished - Apr 2007

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    Whalley, J. M., Ruitenberg, K. M., Sullivan, K., Seshadri, L., Hansen, K., Birch, D., ... Wellington, J. E. (2007). Host cell tropism of equine herpesviruses: Glycoprotein D of EHV-1 enables EHV-4 to infect a non-permissive cell line. Archives of Virology, 152(4), 717-725. https://doi.org/10.1007/s00705-006-0885-x