How specific is my SRM?

The issue of precursor and product ion redundancy

Jamie Sherman, Matthew J. McKay, Keith Ashman, Mark P. Molloy

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Selected reaction monitoring (SRM) MS is proving to be a popular approach for targeted quantitative proteomics. The use of proteotypic peptides as candidates for SRM analysis is a wise first step in SRM method design. The obvious reason for this is the need to avoid redundancy at the sequence level, however this is incidental. The true reason is that homologous peptides result in redundancy in the mass-to-charge domain. This may seem like a trivial subtlety, however, we believe this is an issue of far greater significance than the proteomic community is aware. This VIEWPOINT article serves to highlight the complexity associated with designing SRM assays in light of potential ion redundancy.

Original languageEnglish
Pages (from-to)1120-1123
Number of pages4
JournalProteomics
Volume9
Issue number5
DOIs
Publication statusPublished - Mar 2009

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