How to bring the "unseen" proteome to the limelight via electrophoretic pre-fractionation techniques

Pier Giorgio Righetti*, Annalisa Castagna, Ben Herbert, Giovanni Candiano

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The present review reports a panoply of electrophoretic methods as pre-fractionation tools in proteomic investigations in preparation for mass spectrometry or two-dimensional electrophoresis map analysis. Such electrophoretic pre-fractionation protocols include all those electrokinetic methodologies which are performed in free solution, most of them relying on isoelectric focusing steps (although some approaches based on gels and granulated media are also discussed). Devices associated with electrophoretic separations are multi-chamber apparatuses, such as the multi-compartment electrolyzers equipped with either isoelectric membranes or with isoelectric beads, Off-Gel electrophoresis in a multi-cup device and the Rotofor, an instrument also based on a multi-chamber system but exploiting the conventional technique of carrier-ampholyte-focusing. Other free-flow systems, as well as miniaturized chambers, are also described.

Original languageEnglish
Pages (from-to)3-17
Number of pages15
JournalBioscience Reports
Volume25
Issue number1-2
DOIs
Publication statusPublished - Feb 2005

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