How to build a time-gated luminescence microscope

Dayong Jin, Yiqing Lu, Robert C. Leif, Sean Yang, Megha Rajendran, Lawrence W. Miller

Research output: Contribution to journalArticle

24 Citations (Scopus)


The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.

Original languageEnglish
Article number2.22
Pages (from-to)1-36
Number of pages36
JournalCurrent Protocols in Cytometry
Publication statusPublished - 2 Jan 2014


  • Autofluorescence
  • Lanthanide
  • Microscopy
  • Time-gated luminescence


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