HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents

Jan Stariat; Petra Kovarikova; Jiri Klimes; David B. Lovejoy; Danuta S. Kalinowski; Des R. Richardson

doi:10.1016/j.jchromb.2008.11.044

HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents

Jan Stariat, Petra Kovarikova^*, Jiri Klimes, David B. Lovejoy, Danuta S. Kalinowski, Des R. Richardson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The aim of this study was to develop and validate HPLC methods for the determination in plasma of two novel thiosemicarbazone anti-tumour drugs developed in our laboratories (Dp44mT and N4mT). The appropriate separations were achieved using a HS F5 HPLC column with the mobile phase composed of a mixture of either acetate buffer/EDTA or EDTA and acetonitrile (62:38 and 50:50, v/v, respectively). The plasma samples were pretreated with SPE (phenyl and C18, respectively). Furthermore, these methods were successfully applied to in vitro plasma stability experiments. The investigation has clearly shown that both thiosemicarbazones are markedly more stable in plasma than their aroylhydrazone forerunners. (c) 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	316-322
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	3
DOIs	https://doi.org/10.1016/j.jchromb.2008.11.044
Publication status	Published - 15 Jan 2009
Externally published	Yes

Keywords

Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) 2-hydroxy-1-naphthylaldehyde-4-methyl-3-thiosemicarbazone (N4mT)
Thiosemicarbazone
Stability
HPLC
ISONICOTINOYL HYDRAZONE CLASS
SELECTIVE ANTITUMOR-ACTIVITY
IRON CHELATORS
RABBIT PLASMA
POTENT
THERAPY
ANALOGS
CANCER
VIVO
CELL

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10.1016/j.jchromb.2008.11.044

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Stariat, J., Kovarikova, P., Klimes, J., Lovejoy, D. B., Kalinowski, D. S., & Richardson, D. R. (2009). HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(3), 316-322. https://doi.org/10.1016/j.jchromb.2008.11.044

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title = "HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents",

abstract = "The aim of this study was to develop and validate HPLC methods for the determination in plasma of two novel thiosemicarbazone anti-tumour drugs developed in our laboratories (Dp44mT and N4mT). The appropriate separations were achieved using a HS F5 HPLC column with the mobile phase composed of a mixture of either acetate buffer/EDTA or EDTA and acetonitrile (62:38 and 50:50, v/v, respectively). The plasma samples were pretreated with SPE (phenyl and C18, respectively). Furthermore, these methods were successfully applied to in vitro plasma stability experiments. The investigation has clearly shown that both thiosemicarbazones are markedly more stable in plasma than their aroylhydrazone forerunners. (c) 2008 Elsevier B.V. All rights reserved.",

keywords = "Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) 2-hydroxy-1-naphthylaldehyde-4-methyl-3-thiosemicarbazone (N4mT), Thiosemicarbazone, Stability, HPLC, ISONICOTINOYL HYDRAZONE CLASS, SELECTIVE ANTITUMOR-ACTIVITY, IRON CHELATORS, RABBIT PLASMA, POTENT, THERAPY, ANALOGS, CANCER, VIVO, CELL",

author = "Jan Stariat and Petra Kovarikova and Jiri Klimes and Lovejoy, {David B.} and Kalinowski, {Danuta S.} and Richardson, {Des R.}",

year = "2009",

month = jan,

day = "15",

doi = "10.1016/j.jchromb.2008.11.044",

language = "English",

volume = "877",

pages = "316--322",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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publisher = "Elsevier",

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Stariat, J, Kovarikova, P, Klimes, J, Lovejoy, DB, Kalinowski, DS & Richardson, DR 2009, 'HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 3, pp. 316-322. https://doi.org/10.1016/j.jchromb.2008.11.044

HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents. / Stariat, Jan; Kovarikova, Petra; Klimes, Jiri et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 3, 15.01.2009, p. 316-322.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents

AU - Stariat, Jan

AU - Kovarikova, Petra

AU - Klimes, Jiri

AU - Lovejoy, David B.

AU - Kalinowski, Danuta S.

AU - Richardson, Des R.

PY - 2009/1/15

Y1 - 2009/1/15

N2 - The aim of this study was to develop and validate HPLC methods for the determination in plasma of two novel thiosemicarbazone anti-tumour drugs developed in our laboratories (Dp44mT and N4mT). The appropriate separations were achieved using a HS F5 HPLC column with the mobile phase composed of a mixture of either acetate buffer/EDTA or EDTA and acetonitrile (62:38 and 50:50, v/v, respectively). The plasma samples were pretreated with SPE (phenyl and C18, respectively). Furthermore, these methods were successfully applied to in vitro plasma stability experiments. The investigation has clearly shown that both thiosemicarbazones are markedly more stable in plasma than their aroylhydrazone forerunners. (c) 2008 Elsevier B.V. All rights reserved.

AB - The aim of this study was to develop and validate HPLC methods for the determination in plasma of two novel thiosemicarbazone anti-tumour drugs developed in our laboratories (Dp44mT and N4mT). The appropriate separations were achieved using a HS F5 HPLC column with the mobile phase composed of a mixture of either acetate buffer/EDTA or EDTA and acetonitrile (62:38 and 50:50, v/v, respectively). The plasma samples were pretreated with SPE (phenyl and C18, respectively). Furthermore, these methods were successfully applied to in vitro plasma stability experiments. The investigation has clearly shown that both thiosemicarbazones are markedly more stable in plasma than their aroylhydrazone forerunners. (c) 2008 Elsevier B.V. All rights reserved.

KW - Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) 2-hydroxy-1-naphthylaldehyde-4-methyl-3-thiosemicarbazone (N4mT)

KW - Thiosemicarbazone

KW - Stability

KW - HPLC

KW - ISONICOTINOYL HYDRAZONE CLASS

KW - SELECTIVE ANTITUMOR-ACTIVITY

KW - IRON CHELATORS

KW - RABBIT PLASMA

KW - POTENT

KW - THERAPY

KW - ANALOGS

KW - CANCER

KW - VIVO

KW - CELL

U2 - 10.1016/j.jchromb.2008.11.044

DO - 10.1016/j.jchromb.2008.11.044

M3 - Article

C2 - 19097949

SN - 1570-0232

VL - 877

SP - 316

EP - 322

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 3

ER -

Stariat J, Kovarikova P, Klimes J, Lovejoy DB, Kalinowski DS, Richardson DR. HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 Jan 15;877(3):316-322. doi: 10.1016/j.jchromb.2008.11.044

HPLC methods for determination of two novel thiosemicarbazone anti-cancer drugs (N4mT and Dp44mT) in plasma and their application to in vitro plasma stability of these agents

Abstract

Keywords

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