Human disease glycomics: technology advances enabling protein glycosylation analysis – part 1

Arun V. Everest-Dass, Edward S.X. Moh, Christopher Ashwood, Abdulrahman M.M. Shathili, Nicolle H. Packer

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

Introduction: Protein glycosylation is recognized as an important post-translational modification, with specific substructures having significant effects on protein folding, conformation, distribution, stability and activity. However, due to the structural complexity of glycans, elucidating glycan structure-function relationships is demanding. The fine detail of glycan structures attached to proteins (including sequence, branching, linkage and anomericity) is still best analysed after the glycans are released from the purified or mixture of glycoproteins (glycomics). The technologies currently available for glycomics are becoming streamlined and standardized and many features of protein glycosylation can now be determined using instruments available in most protein analytical laboratories.

Areas covered: This review focuses on the current glycomics technologies being commonly used for the analysis of the microheterogeneity of monosaccharide composition, sequence, branching and linkage of released N- and O-linked glycans that enable the determination of precise glycan structural determinants presented on secreted proteins and on the surface of all cells.

Expert commentary: Several emerging advances in these technologies enabling glycomics analysis are discussed. The technological and bioinformatics requirements to be able to accurately assign these precise glycan features at biological levels in a disease context are assessed.

LanguageEnglish
Pages165-182
Number of pages18
JournalExpert Review of Proteomics
Volume15
Issue number2
DOIs
Publication statusPublished - 1 Feb 2018

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Glycomics
Glycosylation
Polysaccharides
Technology
Proteins
Protein folding
Protein Conformation
Monosaccharides
Protein Folding
Post Translational Protein Processing
Bioinformatics
Computational Biology
Conformations
Glycoproteins
Membrane Proteins

Cite this

Everest-Dass, Arun V. ; Moh, Edward S.X. ; Ashwood, Christopher ; Shathili, Abdulrahman M.M. ; Packer, Nicolle H. / Human disease glycomics : technology advances enabling protein glycosylation analysis – part 1. In: Expert Review of Proteomics. 2018 ; Vol. 15, No. 2. pp. 165-182.
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Human disease glycomics : technology advances enabling protein glycosylation analysis – part 1. / Everest-Dass, Arun V.; Moh, Edward S.X.; Ashwood, Christopher; Shathili, Abdulrahman M.M.; Packer, Nicolle H.

In: Expert Review of Proteomics, Vol. 15, No. 2, 01.02.2018, p. 165-182.

Research output: Contribution to journalReview articleResearchpeer-review

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