Human lens chemistry - UV filters and cataract

Jasminka Mizdrak, Peter G. Hains, Roger J. W. Truscott, Michael J. Davies, Joanne F. Jamie

Research output: Chapter in Book/Report/Conference proceedingOther chapter contributionResearch

Abstract

The primate lens contains fluorescent tryptophan derived compounds known as UV filters. These absorb UV light and protect the lens from UV induced photo-damage. The major UV filters are 3-hydroxykynurenine O-Beta-D-glucoside, 4-(2-amino-3-hydroxyphenyl)-4-oxobutanoic acid O-Beta-D-glucoside, kynurenine and 3-hydroxykynurenine. As the lens ages, a broad spectrum of molecular and biochemical changes occurs. We have shown that the UV filter compounds at physiological pH undergo spontaneous deamination, and bind to lens proteins and lens nucleophiles.(1,2) We have also demonstrated that kynurenine bound to lens proteins become highly susceptible to photo-oxidation in the presence of UV light and generates protein damage.(3) A greater understanding of the UV protective and photosensitising mechanisms of known human lens UV filters, and determination of as yet unidentified UV filters, is therefore required. The identification, synthesis and properties of human lens UV filters will be discussed. 1) Vazquez S., et al., J. Biol. Chem., 277, 4867-4873 (2002) 2) Hood B.D., et al., J. Biol. Chem., 274, 32547-32550 (1999) 3) Parker N.R., et al., Free Radical Biol. & Med., 37, 1479-1489 (2004)
LanguageEnglish
Title of host publication1st European chemistry congress
Subtitle of host publicationabstract book
Place of PublicationHungary
PublisherEuropean Association for Chemical and Molecular Sciences
Pages227
Number of pages1
ISBN (Print)9639319619
Publication statusPublished - 2006
EventEuropean Chemistry Congress (1st : 2006) - Budapest, Hungary
Duration: 27 Aug 200631 Aug 2006

Conference

ConferenceEuropean Chemistry Congress (1st : 2006)
CityBudapest, Hungary
Period27/08/0631/08/06

Fingerprint

Lenses
Kynurenine
Crystallins
Ultraviolet radiation
Nucleophiles
Photooxidation
Glucosides
Tryptophan
Free Radicals
Proteins
3-hydroxykynurenine

Keywords

  • UV filter
  • cataract
  • human lens

Cite this

Mizdrak, J., Hains, P. G., Truscott, R. J. W., Davies, M. J., & Jamie, J. F. (2006). Human lens chemistry - UV filters and cataract. In 1st European chemistry congress: abstract book (pp. 227). Hungary: European Association for Chemical and Molecular Sciences.
Mizdrak, Jasminka ; Hains, Peter G. ; Truscott, Roger J. W. ; Davies, Michael J. ; Jamie, Joanne F. / Human lens chemistry - UV filters and cataract. 1st European chemistry congress: abstract book. Hungary : European Association for Chemical and Molecular Sciences, 2006. pp. 227
@inbook{8bdd2107f4724df799f8d93b60f7ee72,
title = "Human lens chemistry - UV filters and cataract",
abstract = "The primate lens contains fluorescent tryptophan derived compounds known as UV filters. These absorb UV light and protect the lens from UV induced photo-damage. The major UV filters are 3-hydroxykynurenine O-Beta-D-glucoside, 4-(2-amino-3-hydroxyphenyl)-4-oxobutanoic acid O-Beta-D-glucoside, kynurenine and 3-hydroxykynurenine. As the lens ages, a broad spectrum of molecular and biochemical changes occurs. We have shown that the UV filter compounds at physiological pH undergo spontaneous deamination, and bind to lens proteins and lens nucleophiles.(1,2) We have also demonstrated that kynurenine bound to lens proteins become highly susceptible to photo-oxidation in the presence of UV light and generates protein damage.(3) A greater understanding of the UV protective and photosensitising mechanisms of known human lens UV filters, and determination of as yet unidentified UV filters, is therefore required. The identification, synthesis and properties of human lens UV filters will be discussed. 1) Vazquez S., et al., J. Biol. Chem., 277, 4867-4873 (2002) 2) Hood B.D., et al., J. Biol. Chem., 274, 32547-32550 (1999) 3) Parker N.R., et al., Free Radical Biol. & Med., 37, 1479-1489 (2004)",
keywords = "UV filter, cataract, human lens",
author = "Jasminka Mizdrak and Hains, {Peter G.} and Truscott, {Roger J. W.} and Davies, {Michael J.} and Jamie, {Joanne F.}",
year = "2006",
language = "English",
isbn = "9639319619",
pages = "227",
booktitle = "1st European chemistry congress",
publisher = "European Association for Chemical and Molecular Sciences",

}

Mizdrak, J, Hains, PG, Truscott, RJW, Davies, MJ & Jamie, JF 2006, Human lens chemistry - UV filters and cataract. in 1st European chemistry congress: abstract book. European Association for Chemical and Molecular Sciences, Hungary, pp. 227, European Chemistry Congress (1st : 2006), Budapest, Hungary, 27/08/06.

Human lens chemistry - UV filters and cataract. / Mizdrak, Jasminka; Hains, Peter G.; Truscott, Roger J. W.; Davies, Michael J.; Jamie, Joanne F.

1st European chemistry congress: abstract book. Hungary : European Association for Chemical and Molecular Sciences, 2006. p. 227.

Research output: Chapter in Book/Report/Conference proceedingOther chapter contributionResearch

TY - CHAP

T1 - Human lens chemistry - UV filters and cataract

AU - Mizdrak, Jasminka

AU - Hains, Peter G.

AU - Truscott, Roger J. W.

AU - Davies, Michael J.

AU - Jamie, Joanne F.

PY - 2006

Y1 - 2006

N2 - The primate lens contains fluorescent tryptophan derived compounds known as UV filters. These absorb UV light and protect the lens from UV induced photo-damage. The major UV filters are 3-hydroxykynurenine O-Beta-D-glucoside, 4-(2-amino-3-hydroxyphenyl)-4-oxobutanoic acid O-Beta-D-glucoside, kynurenine and 3-hydroxykynurenine. As the lens ages, a broad spectrum of molecular and biochemical changes occurs. We have shown that the UV filter compounds at physiological pH undergo spontaneous deamination, and bind to lens proteins and lens nucleophiles.(1,2) We have also demonstrated that kynurenine bound to lens proteins become highly susceptible to photo-oxidation in the presence of UV light and generates protein damage.(3) A greater understanding of the UV protective and photosensitising mechanisms of known human lens UV filters, and determination of as yet unidentified UV filters, is therefore required. The identification, synthesis and properties of human lens UV filters will be discussed. 1) Vazquez S., et al., J. Biol. Chem., 277, 4867-4873 (2002) 2) Hood B.D., et al., J. Biol. Chem., 274, 32547-32550 (1999) 3) Parker N.R., et al., Free Radical Biol. & Med., 37, 1479-1489 (2004)

AB - The primate lens contains fluorescent tryptophan derived compounds known as UV filters. These absorb UV light and protect the lens from UV induced photo-damage. The major UV filters are 3-hydroxykynurenine O-Beta-D-glucoside, 4-(2-amino-3-hydroxyphenyl)-4-oxobutanoic acid O-Beta-D-glucoside, kynurenine and 3-hydroxykynurenine. As the lens ages, a broad spectrum of molecular and biochemical changes occurs. We have shown that the UV filter compounds at physiological pH undergo spontaneous deamination, and bind to lens proteins and lens nucleophiles.(1,2) We have also demonstrated that kynurenine bound to lens proteins become highly susceptible to photo-oxidation in the presence of UV light and generates protein damage.(3) A greater understanding of the UV protective and photosensitising mechanisms of known human lens UV filters, and determination of as yet unidentified UV filters, is therefore required. The identification, synthesis and properties of human lens UV filters will be discussed. 1) Vazquez S., et al., J. Biol. Chem., 277, 4867-4873 (2002) 2) Hood B.D., et al., J. Biol. Chem., 274, 32547-32550 (1999) 3) Parker N.R., et al., Free Radical Biol. & Med., 37, 1479-1489 (2004)

KW - UV filter

KW - cataract

KW - human lens

M3 - Other chapter contribution

SN - 9639319619

SP - 227

BT - 1st European chemistry congress

PB - European Association for Chemical and Molecular Sciences

CY - Hungary

ER -

Mizdrak J, Hains PG, Truscott RJW, Davies MJ, Jamie JF. Human lens chemistry - UV filters and cataract. In 1st European chemistry congress: abstract book. Hungary: European Association for Chemical and Molecular Sciences. 2006. p. 227