TY - JOUR
T1 - Human proteome project and human pluripotent stem cells
T2 - odd bedfellows or a perfect match?
AU - Alikhani, Mehdi
AU - Karamzadeh, Razieh
AU - Rahimi, Pardis
AU - Adib, Samane
AU - Baharvand, Hossein
AU - Salekdeh, Ghasem Hosseini
PY - 2020/12/4
Y1 - 2020/12/4
N2 - The Chromosome-Centric Human Proteome Project (C-HPP) aims at the identification of missing proteins (MPs) and the functional characterization of functionally unannotated PE1 (uPE1) proteins. A major challenge in addressing this goal is that many human proteins and MPs are silent in adult cells. A promising approach to overcome such challenge is to exploit the advantage of novel tools such as pluripotent stem cells (PSCs), which are capable of differentiation into three embryonic germ layers, namely, the endoderm, mesoderm, and ectoderm. Here we present several examples of how the Human Y Chromosome Proteome Project (Y-HPP) benefited from this approach to meet C-HPP goals. Furthermore, we discuss how integrating CRISPR engineering, human-induced pluripotent stem cell (hiPSC)-derived disease modeling systems, and organoid technologies provides a unique platform for Y-HPP and C-HPP for MP identification and the functional characterization of human proteins, especially uPE1s.
AB - The Chromosome-Centric Human Proteome Project (C-HPP) aims at the identification of missing proteins (MPs) and the functional characterization of functionally unannotated PE1 (uPE1) proteins. A major challenge in addressing this goal is that many human proteins and MPs are silent in adult cells. A promising approach to overcome such challenge is to exploit the advantage of novel tools such as pluripotent stem cells (PSCs), which are capable of differentiation into three embryonic germ layers, namely, the endoderm, mesoderm, and ectoderm. Here we present several examples of how the Human Y Chromosome Proteome Project (Y-HPP) benefited from this approach to meet C-HPP goals. Furthermore, we discuss how integrating CRISPR engineering, human-induced pluripotent stem cell (hiPSC)-derived disease modeling systems, and organoid technologies provides a unique platform for Y-HPP and C-HPP for MP identification and the functional characterization of human proteins, especially uPE1s.
KW - Chromosome-Centric Human Proteome Project (C-HPP)
KW - missing proteins (MPs)
KW - functionally unannotated PE1 proteins (uPE1s)
KW - pluripotent stem cells (PSCs)
KW - CRISPR
KW - organoid
UR - http://www.scopus.com/inward/record.url?scp=85095851284&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.0c00689
DO - 10.1021/acs.jproteome.0c00689
M3 - Article
C2 - 33124832
AN - SCOPUS:85095851284
SN - 1535-3893
VL - 19
SP - 4747
EP - 4753
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 12
ER -