Human proteome project and human pluripotent stem cells: odd bedfellows or a perfect match?

Mehdi Alikhani, Razieh Karamzadeh, Pardis Rahimi, Samane Adib, Hossein Baharvand*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)


    The Chromosome-Centric Human Proteome Project (C-HPP) aims at the identification of missing proteins (MPs) and the functional characterization of functionally unannotated PE1 (uPE1) proteins. A major challenge in addressing this goal is that many human proteins and MPs are silent in adult cells. A promising approach to overcome such challenge is to exploit the advantage of novel tools such as pluripotent stem cells (PSCs), which are capable of differentiation into three embryonic germ layers, namely, the endoderm, mesoderm, and ectoderm. Here we present several examples of how the Human Y Chromosome Proteome Project (Y-HPP) benefited from this approach to meet C-HPP goals. Furthermore, we discuss how integrating CRISPR engineering, human-induced pluripotent stem cell (hiPSC)-derived disease modeling systems, and organoid technologies provides a unique platform for Y-HPP and C-HPP for MP identification and the functional characterization of human proteins, especially uPE1s.

    Original languageEnglish
    Pages (from-to)4747-4753
    Number of pages7
    JournalJournal of Proteome Research
    Issue number12
    Publication statusPublished - 4 Dec 2020


    • Chromosome-Centric Human Proteome Project (C-HPP)
    • missing proteins (MPs)
    • functionally unannotated PE1 proteins (uPE1s)
    • pluripotent stem cells (PSCs)
    • CRISPR
    • organoid


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