Humoral opsonins of the tunicate, Pyura stolonifera

Sarina Pearce, Rebecca A. Newton, Sham V. Nair, David A. Raftos*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    This study characterizes humoral opsonins from the tunicate, Pyura stolonifera. The predominant opsonic components in P. stolonifera hemolymph were found to be calcium-dependent lectins with broad carbohydrate specificities. The opsonic lectins were purified by carbohydrate affinity chromatography which eluted a complex pattern of proteins ranging in molecular mass from 80 to >200kDa. Reducing and two dimensional SDS-PAGE indicated that the diversity of mature lectins evident under non-reducing conditions resulted from the differential oligomerization of two polypeptide sub-units (35 and 22kDa). In addition to lectin-mediated opsonic activity, hemolymph was also found to contain proteolytically activated opsonins. These data suggest that multiple, possibly interactive opsonic systems co-exist in P. stolonifera.

    Original languageEnglish
    Pages (from-to)377-385
    Number of pages9
    JournalDevelopmental and Comparative Immunology
    Volume25
    Issue number5-6
    DOIs
    Publication statusPublished - Jun 2001

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