Hydrogen peroxide modulation of the respiratory burst of human neutrophils

Jacqueline S. Winn; Jennifer Guille; Janusz M. Gebicki; Richard O. Day

doi:10.1016/0006-2952(91)90007-R

Hydrogen peroxide modulation of the respiratory burst of human neutrophils

Jacqueline S. Winn^*, Jennifer Guille, Janusz M. Gebicki, Richard O. Day

^*Corresponding author for this work

Faculty of Science and Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Addition of micromolar concentrations of hydrogen peroxide (H₂O₂) to human neutrophils resulted in a dose-dependent luminol-enhanced chemiluminescent response. Pretreatment of neutrophils with micromolar concentrations of H₂O₂ altered their response to the surface acting stimulants serumtreated zymosan (STZ) and formyl-methionyl-leucyl-phenylalanine (fMLP), but not to the intracellular stimulant phorbol myristate acetate (PMA). The alterations were partially reversible by catalase, but exacerbated by Superoxide dismutase. These results suggest a modulatory role for H₂O₂ in the respiratory burst of neutrophils.

Original language	English
Pages (from-to)	31-36
Number of pages	6
Journal	Biochemical Pharmacology
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0006-2952(91)90007-R
Publication status	Published - 1 Jan 1991

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title = "Hydrogen peroxide modulation of the respiratory burst of human neutrophils",

abstract = "Addition of micromolar concentrations of hydrogen peroxide (H2O2) to human neutrophils resulted in a dose-dependent luminol-enhanced chemiluminescent response. Pretreatment of neutrophils with micromolar concentrations of H2O2 altered their response to the surface acting stimulants serumtreated zymosan (STZ) and formyl-methionyl-leucyl-phenylalanine (fMLP), but not to the intracellular stimulant phorbol myristate acetate (PMA). The alterations were partially reversible by catalase, but exacerbated by Superoxide dismutase. These results suggest a modulatory role for H2O2 in the respiratory burst of neutrophils.",

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AU - Winn, Jacqueline S.

AU - Guille, Jennifer

AU - Gebicki, Janusz M.

AU - Day, Richard O.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Addition of micromolar concentrations of hydrogen peroxide (H2O2) to human neutrophils resulted in a dose-dependent luminol-enhanced chemiluminescent response. Pretreatment of neutrophils with micromolar concentrations of H2O2 altered their response to the surface acting stimulants serumtreated zymosan (STZ) and formyl-methionyl-leucyl-phenylalanine (fMLP), but not to the intracellular stimulant phorbol myristate acetate (PMA). The alterations were partially reversible by catalase, but exacerbated by Superoxide dismutase. These results suggest a modulatory role for H2O2 in the respiratory burst of neutrophils.

AB - Addition of micromolar concentrations of hydrogen peroxide (H2O2) to human neutrophils resulted in a dose-dependent luminol-enhanced chemiluminescent response. Pretreatment of neutrophils with micromolar concentrations of H2O2 altered their response to the surface acting stimulants serumtreated zymosan (STZ) and formyl-methionyl-leucyl-phenylalanine (fMLP), but not to the intracellular stimulant phorbol myristate acetate (PMA). The alterations were partially reversible by catalase, but exacerbated by Superoxide dismutase. These results suggest a modulatory role for H2O2 in the respiratory burst of neutrophils.

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SP - 31

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JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 1

ER -

Hydrogen peroxide modulation of the respiratory burst of human neutrophils

Abstract

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