Abstract
Rationale and objectives: In conscious mammals, hypoxia produces respiratory alkalosis which is assumed to silence central respiratory chemoreceptors (CRCs). We tested this theory by examining how hypoxia changes the activity of the RTN, a likely CRC and chemoreflex integrator.
Methods: RTN and nearby C1 neurons were bilaterally transduced with archaerhodopsin using a lentiviral vector (PRSx8-ArchT-EYFP-LVV). We first showed that RTN neurons were silenced by green light in anesthetized rats. We then recorded the effect of RTN inhibition (10s) on breathing (fR, VT), BP, EEG and neck EMG in conscious rats exposed to normoxia, hypocapnic hypoxia (12 or 15% O2), isocapnic hypoxia (12% O2 + 3% CO2) or hypoxia in presence of acetazolamide (ACTZ). Arterial PO2, PCO2, pH and HCO3 were measured in each condition.
Results: In normoxia RTN inhibition reduced breathing equally during non-REM sleep and quiet wake and BP was unchanged. ΔfR was largest in normoxia, reduced in 15% O2, nul in 12% O2 and back to control (normoxia level) in normocapnic hypoxia.ΔVT followed the same trend. RTN inhibition caused much larger fR and VT reductions in ACTZ-treated rats. Overall the bradypnea caused by RTN inhibition was linearly correlated with pHa (p < 0.0002). Above pHa 7.5, no fR change was elicited.
Conclusions: RTN neurons regulate breathing equally during sleep and wake. RTN neurons are the first putative CRCs demonstrably silenced by hypocapnic hypoxia in conscious mammals. RTN is silent above pHa 7.5 and increasingly active below this value. Under hypocapnic hypoxia carotid body stimulation drives breathing via pathways that bypass RTN. ACTZ increases breathing by activating RTN. Funding: HL074011 (PGG), 5T32HL007284-38 (TMB).
Methods: RTN and nearby C1 neurons were bilaterally transduced with archaerhodopsin using a lentiviral vector (PRSx8-ArchT-EYFP-LVV). We first showed that RTN neurons were silenced by green light in anesthetized rats. We then recorded the effect of RTN inhibition (10s) on breathing (fR, VT), BP, EEG and neck EMG in conscious rats exposed to normoxia, hypocapnic hypoxia (12 or 15% O2), isocapnic hypoxia (12% O2 + 3% CO2) or hypoxia in presence of acetazolamide (ACTZ). Arterial PO2, PCO2, pH and HCO3 were measured in each condition.
Results: In normoxia RTN inhibition reduced breathing equally during non-REM sleep and quiet wake and BP was unchanged. ΔfR was largest in normoxia, reduced in 15% O2, nul in 12% O2 and back to control (normoxia level) in normocapnic hypoxia.ΔVT followed the same trend. RTN inhibition caused much larger fR and VT reductions in ACTZ-treated rats. Overall the bradypnea caused by RTN inhibition was linearly correlated with pHa (p < 0.0002). Above pHa 7.5, no fR change was elicited.
Conclusions: RTN neurons regulate breathing equally during sleep and wake. RTN neurons are the first putative CRCs demonstrably silenced by hypocapnic hypoxia in conscious mammals. RTN is silent above pHa 7.5 and increasingly active below this value. Under hypocapnic hypoxia carotid body stimulation drives breathing via pathways that bypass RTN. ACTZ increases breathing by activating RTN. Funding: HL074011 (PGG), 5T32HL007284-38 (TMB).
Original language | English |
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Article number | 1051.2 |
Journal | FASEB Journal |
Volume | 29 |
Issue number | 1 supplement |
Publication status | Published - Apr 2015 |
Externally published | Yes |
Event | Experimental Biology Meeting - Boston, United States Duration: 28 Mar 2015 → 1 Apr 2015 |