Arabidopsis GERANYLGERANYL DIPHOSPHATE SYNTHASE 11 is a hub isozyme required for the production of most photosynthesis-related isoprenoids

M. Águila Ruiz-Sola, Diana Coman, Gilles Beck, M. Victoria Barja, Maite Colinas, Alexander Graf, Ralf Welsch, Philipp Rütimann, Peter Bühlmann, Laurent Bigler, Wilhelm Gruissem, Manuel Rodríguez-Concepción*, Eva Vranová*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)

Abstract

• Most plastid isoprenoids, including photosynthesis-related metabolites such as carotenoids and the side chain of chlorophylls, tocopherols (vitamin E), phylloquinones (vitamin K), and plastoquinones, derive from geranylgeranyl diphosphate (GGPP) synthesized by GGPP synthase (GGPPS) enzymes. Seven out of 10 functional GGPPS isozymes in Arabidopsis thaliana reside in plastids. We aimed to address the function of different GGPPS paralogues for plastid isoprenoid biosynthesis. 

• We constructed a gene co-expression network (GCN) using GGPPS paralogues as guide genes and genes from the upstream and downstream pathways as query genes. Furthermore, knock-out and/or knock-down ggpps mutants were generated and their growth and metabolic phenotypes were analyzed. Also, interacting protein partners of GGPPS11 were searched for. 

• Our data showed that GGPPS11, encoding the only plastid isozyme essential for plant development, functions as a hub gene among GGPPS paralogues and is required for the production of all major groups of plastid isoprenoids. Furthermore, we showed that the GGPPS11 protein physically interacts with enzymes that use GGPP for the production of carotenoids, chlorophylls, tocopherols, phylloquinone, and plastoquinone. 

• GGPPS11 is a hub isozyme required for the production of most photosynthesis-related isoprenoids. Both gene co-expression and protein-protein interaction likely contribute to the channeling of GGPP by GGPPS11.

Original languageEnglish
Pages (from-to)252-264
Number of pages13
JournalNew Phytologist
Volume209
Issue number1
Early online date30 Jul 2015
DOIs
Publication statusPublished - Jan 2016
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Gene paralogues
  • Geranylgeranyl diphosphate synthase
  • GGPPS11

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