Cryptosporidium fayeri: diversity within the GP60 locus of isolates from different marsupial hosts

Michelle L. Power, Cristel Cheung-Kwok-Sang, Martin Slade, Sally Williamson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The highly polymorphic 60 kDa glycoprotein (GP60) of Cryptosporidium is an important tool for investigating the epidemiology of this parasite. Characterization of the GP60 gene has only been performed for 3 of the 20 known Cryptosporidium species, and has already enabled sub-typing and source tracking of species with human significance. We have characterised a fourth species, Cryptosporidium fayeri, at the GP60 locus using isolates (n = 26) from different marsupial hosts to assess the diversity of GP60 within this species. The analysis demonstrated that C. fayeri isolates could be assigned to 6 subtypes which were associated with host species and locality. The intra-species diversity for the host-adapted C. fayeri was less than the diversity for human pathogenic species suggesting that the GP60 locus is under less selective pressure in these than host-adapted species.

LanguageEnglish
Pages219-223
Number of pages5
JournalExperimental Parasitology
Volume121
Issue number3
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Marsupialia
Cryptosporidium
Glycoproteins
Parasites
Epidemiology
Genes

Keywords

  • apicomplexa
  • Cryptosporidiumfayeri
  • marsupials
  • sporozoite
  • surface antigens
  • GP60

Cite this

Power, Michelle L. ; Cheung-Kwok-Sang, Cristel ; Slade, Martin ; Williamson, Sally. / Cryptosporidium fayeri : diversity within the GP60 locus of isolates from different marsupial hosts. In: Experimental Parasitology. 2009 ; Vol. 121, No. 3. pp. 219-223.
@article{6a5f06aa09e74371b50ba9018c87600f,
title = "Cryptosporidium fayeri: diversity within the GP60 locus of isolates from different marsupial hosts",
abstract = "The highly polymorphic 60 kDa glycoprotein (GP60) of Cryptosporidium is an important tool for investigating the epidemiology of this parasite. Characterization of the GP60 gene has only been performed for 3 of the 20 known Cryptosporidium species, and has already enabled sub-typing and source tracking of species with human significance. We have characterised a fourth species, Cryptosporidium fayeri, at the GP60 locus using isolates (n = 26) from different marsupial hosts to assess the diversity of GP60 within this species. The analysis demonstrated that C. fayeri isolates could be assigned to 6 subtypes which were associated with host species and locality. The intra-species diversity for the host-adapted C. fayeri was less than the diversity for human pathogenic species suggesting that the GP60 locus is under less selective pressure in these than host-adapted species.",
keywords = "apicomplexa, Cryptosporidiumfayeri, marsupials, sporozoite, surface antigens, GP60",
author = "Power, {Michelle L.} and Cristel Cheung-Kwok-Sang and Martin Slade and Sally Williamson",
year = "2009",
month = "3",
doi = "10.1016/j.exppara.2008.10.016",
language = "English",
volume = "121",
pages = "219--223",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "3",

}

Cryptosporidium fayeri : diversity within the GP60 locus of isolates from different marsupial hosts. / Power, Michelle L.; Cheung-Kwok-Sang, Cristel; Slade, Martin; Williamson, Sally.

In: Experimental Parasitology, Vol. 121, No. 3, 03.2009, p. 219-223.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Cryptosporidium fayeri

T2 - Experimental Parasitology

AU - Power, Michelle L.

AU - Cheung-Kwok-Sang, Cristel

AU - Slade, Martin

AU - Williamson, Sally

PY - 2009/3

Y1 - 2009/3

N2 - The highly polymorphic 60 kDa glycoprotein (GP60) of Cryptosporidium is an important tool for investigating the epidemiology of this parasite. Characterization of the GP60 gene has only been performed for 3 of the 20 known Cryptosporidium species, and has already enabled sub-typing and source tracking of species with human significance. We have characterised a fourth species, Cryptosporidium fayeri, at the GP60 locus using isolates (n = 26) from different marsupial hosts to assess the diversity of GP60 within this species. The analysis demonstrated that C. fayeri isolates could be assigned to 6 subtypes which were associated with host species and locality. The intra-species diversity for the host-adapted C. fayeri was less than the diversity for human pathogenic species suggesting that the GP60 locus is under less selective pressure in these than host-adapted species.

AB - The highly polymorphic 60 kDa glycoprotein (GP60) of Cryptosporidium is an important tool for investigating the epidemiology of this parasite. Characterization of the GP60 gene has only been performed for 3 of the 20 known Cryptosporidium species, and has already enabled sub-typing and source tracking of species with human significance. We have characterised a fourth species, Cryptosporidium fayeri, at the GP60 locus using isolates (n = 26) from different marsupial hosts to assess the diversity of GP60 within this species. The analysis demonstrated that C. fayeri isolates could be assigned to 6 subtypes which were associated with host species and locality. The intra-species diversity for the host-adapted C. fayeri was less than the diversity for human pathogenic species suggesting that the GP60 locus is under less selective pressure in these than host-adapted species.

KW - apicomplexa

KW - Cryptosporidiumfayeri

KW - marsupials

KW - sporozoite

KW - surface antigens

KW - GP60

UR - http://www.scopus.com/inward/record.url?scp=60049088248&partnerID=8YFLogxK

U2 - 10.1016/j.exppara.2008.10.016

DO - 10.1016/j.exppara.2008.10.016

M3 - Article

VL - 121

SP - 219

EP - 223

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3

ER -