Identification of 13,14‐dihydro‐15‐oxo‐prostaglandin F in the circulation during infusions of bradykinin and prostaglandin E2 in man

S. E. Barrow, J. Cockcroft, C. T. Dollery, N. E. Hickling, J. M. Ritter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Bradykinin, prostaglandin E2 (PGE2), PGD2 and vehicle (saline) were each administered intravenously on separate occasions to 6 healthy men for a period of 60 min. 13,14‐Dihydro‐15‐oxo‐PGF was identified in plasma samples obtained during intravenous infusions of bradykinin and PGE2 but not during infusions of PGD2 or saline. The structure of this metabolite was verified by comparison of three different derivatives with authentic standards, using gas chromatography/electron capture mass spectrometry. Bradykinin increased plasma concentrations of 13,14‐dihydro‐15‐oxo‐PGF from baseline values in the range < 5–10 pg ml−1 to 28–403 pg ml−1. PGE2 increased plasma concentrations of 13,14‐dihydro‐15‐oxo‐PGF from baseline values in the range < 5–17 pg ml−1 to 160–603 pg ml−1. Neither PGD2 nor the vehicle affected 13,14‐dihydro‐15‐oxo‐PGF concentrations. We conclude that bradykinin‐stimulated 13,14‐dihydro‐15‐oxo‐PGF may be derived from PGE2 or PGF. The possibility that these prostaglandins are synthesized by stimulation of microvascular endothelium during bradykinin infusion is discussed. 1987 British Pharmacological Society

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalBritish Journal of Pharmacology
Volume91
Issue number1
DOIs
Publication statusPublished - 1987

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