By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.
- Mass spectrometry