Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

C. Tsui, L. M. Shao, C. M. Wang, G. Q. Tao, D. S. Letham*, C. W. Parker, R. E. Summons, C. H. Hocart

*Corresponding author for this work

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

    Original languageEnglish
    Pages (from-to)225-231
    Number of pages7
    JournalPlant Science Letters
    Volume32
    Issue number1-2
    DOIs
    Publication statusPublished - 1983

    Keywords

    • Corms
    • Cytokinin
    • Eleocharis
    • Isopentenyladenosine
    • Mass spectrometry

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  • Cite this

    Tsui, C., Shao, L. M., Wang, C. M., Tao, G. Q., Letham, D. S., Parker, C. W., ... Hocart, C. H. (1983). Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa). Plant Science Letters, 32(1-2), 225-231. https://doi.org/10.1016/0304-4211(83)90119-0