Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

C. Tsui, L. M. Shao, C. M. Wang, G. Q. Tao, D. S. Letham*, C. W. Parker, R. E. Summons, C. H. Hocart

*Corresponding author for this work

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Abstract

By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalPlant Science Letters
Volume32
Issue number1-2
DOIs
Publication statusPublished - 1983

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Keywords

  • Corms
  • Cytokinin
  • Eleocharis
  • Isopentenyladenosine
  • Mass spectrometry

Cite this

Tsui, C., Shao, L. M., Wang, C. M., Tao, G. Q., Letham, D. S., Parker, C. W., ... Hocart, C. H. (1983). Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa). Plant Science Letters, 32(1-2), 225-231. https://doi.org/10.1016/0304-4211(83)90119-0