Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

C. Tsui, L. M. Shao, C. M. Wang, G. Q. Tao, D. S. Letham*, C. W. Parker, R. E. Summons, C. H. Hocart

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)


    By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

    Original languageEnglish
    Pages (from-to)225-231
    Number of pages7
    JournalPlant Science Letters
    Issue number1-2
    Publication statusPublished - 1983


    • Corms
    • Cytokinin
    • Eleocharis
    • Isopentenyladenosine
    • Mass spectrometry


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