Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

C. Tsui; L. M. Shao; C. M. Wang; G. Q. Tao; D. S. Letham; C. W. Parker; R. E. Summons; C. H. Hocart

doi:10.1016/0304-4211(83)90119-0

Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

C. Tsui, L. M. Shao, C. M. Wang, G. Q. Tao, D. S. Letham^*, C. W. Parker, R. E. Summons, C. H. Hocart

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N⁶ - (Δ²-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Plant Science Letters
Volume	32
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-4211(83)90119-0
Publication status	Published - 1983

Keywords

Corms
Cytokinin
Eleocharis
Isopentenyladenosine
Mass spectrometry

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10.1016/0304-4211(83)90119-0

Cite this

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title = "Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)",

abstract = "By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.",

keywords = "Corms, Cytokinin, Eleocharis, Isopentenyladenosine, Mass spectrometry",

author = "C. Tsui and Shao, \{L. M.\} and Wang, \{C. M.\} and Tao, \{G. Q.\} and Letham, \{D. S.\} and Parker, \{C. W.\} and Summons, \{R. E.\} and Hocart, \{C. H.\}",

year = "1983",

doi = "10.1016/0304-4211(83)90119-0",

language = "English",

volume = "32",

pages = "225--231",

journal = "Plant Science Letters",

issn = "0304-4211",

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number = "1-2",

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TY - JOUR

T1 - Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

AU - Tsui, C.

AU - Shao, L. M.

AU - Wang, C. M.

AU - Tao, G. Q.

AU - Letham, D. S.

AU - Parker, C. W.

AU - Summons, R. E.

AU - Hocart, C. H.

PY - 1983

Y1 - 1983

N2 - By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

AB - By using the soybean callus and Amaranthus betacyanin bioassays to monitor for cytokinin activity, the principal cytokinin in extracts of corms of Eleocharis tuberosa (Roxb.) was purified by a procedure involving sequential chromatography on Dowex-50, silica gel and Sephadex LH-20, and finally reverse phase high pressure liquid chromatography (HPLC). The cytokinin, N6 - (Δ2-isopentenyl)-adenosine (iA), was identified by electron-impact (EI) and chemical-ionization (CI) mass spectrometry (MS) and quantified by gas chromatography (GC)-MS as the trifluoroacetyl derivative. This is the first identification of a cytokinin in a vegetative organ of storage and perennation other than a storage root.

KW - Corms

KW - Cytokinin

KW - Eleocharis

KW - Isopentenyladenosine

KW - Mass spectrometry

UR - https://www.scopus.com/pages/publications/19144366848

U2 - 10.1016/0304-4211(83)90119-0

DO - 10.1016/0304-4211(83)90119-0

M3 - Article

AN - SCOPUS:19144366848

SN - 0304-4211

VL - 32

SP - 225

EP - 231

JO - Plant Science Letters

JF - Plant Science Letters

IS - 1-2

ER -

Identification of a cytokinin in water chestnuts (corms of Eleocharis tuberosa)

Abstract

Keywords

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