Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA-AFLP

C. W. Wee; S. F. Lee; C. Robin; D. G. Heckel

doi:10.1111/j.1365-2583.2008.00809.x

Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA-AFLP

C. W. Wee, S. F. Lee, C. Robin, D. G. Heckel

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

A cDNA-amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera. Larvae and adults of this strain manifest ∼50-fold resistance, which is suppressible by piperonyl butoxide and controlled by the semidominant gene RFen1 previously mapped to AFLP Linkage Group 13. Two cytochrome P450s (CYP337B1 and CYP4S1), one carboxylesterase-like protein and one glutathione transferase were found to be constitutively upregulated in resistant insects. Mapping of these potential detoxification genes showed that one of them, the novel P450 CYP337B1, was tightly linked to the resistance locus. This suggests that the RFen1^R allele has a c/s-acting effect on CYP337B1 expression, and possible tians-acWng effects on expression of other genes.

Original language	English
Pages (from-to)	351-360
Number of pages	10
Journal	Insect Molecular Biology
Volume	17
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-2583.2008.00809.x
Publication status	Published - Aug 2008
Externally published	Yes

Keywords

cDNA-AFLP
Cotton bollworm
Fenvalerate
Linkage mapping
P450

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10.1111/j.1365-2583.2008.00809.x

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title = "Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA-AFLP",

abstract = "A cDNA-amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera. Larvae and adults of this strain manifest ∼50-fold resistance, which is suppressible by piperonyl butoxide and controlled by the semidominant gene RFen1 previously mapped to AFLP Linkage Group 13. Two cytochrome P450s (CYP337B1 and CYP4S1), one carboxylesterase-like protein and one glutathione transferase were found to be constitutively upregulated in resistant insects. Mapping of these potential detoxification genes showed that one of them, the novel P450 CYP337B1, was tightly linked to the resistance locus. This suggests that the RFen1R allele has a c/s-acting effect on CYP337B1 expression, and possible tians-acWng effects on expression of other genes.",

keywords = "cDNA-AFLP, Cotton bollworm, Fenvalerate, Linkage mapping, P450",

author = "Wee, {C. W.} and Lee, {S. F.} and C. Robin and Heckel, {D. G.}",

year = "2008",

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doi = "10.1111/j.1365-2583.2008.00809.x",

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AU - Wee, C. W.

AU - Lee, S. F.

AU - Robin, C.

AU - Heckel, D. G.

PY - 2008/8

Y1 - 2008/8

N2 - A cDNA-amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera. Larvae and adults of this strain manifest ∼50-fold resistance, which is suppressible by piperonyl butoxide and controlled by the semidominant gene RFen1 previously mapped to AFLP Linkage Group 13. Two cytochrome P450s (CYP337B1 and CYP4S1), one carboxylesterase-like protein and one glutathione transferase were found to be constitutively upregulated in resistant insects. Mapping of these potential detoxification genes showed that one of them, the novel P450 CYP337B1, was tightly linked to the resistance locus. This suggests that the RFen1R allele has a c/s-acting effect on CYP337B1 expression, and possible tians-acWng effects on expression of other genes.

AB - A cDNA-amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera. Larvae and adults of this strain manifest ∼50-fold resistance, which is suppressible by piperonyl butoxide and controlled by the semidominant gene RFen1 previously mapped to AFLP Linkage Group 13. Two cytochrome P450s (CYP337B1 and CYP4S1), one carboxylesterase-like protein and one glutathione transferase were found to be constitutively upregulated in resistant insects. Mapping of these potential detoxification genes showed that one of them, the novel P450 CYP337B1, was tightly linked to the resistance locus. This suggests that the RFen1R allele has a c/s-acting effect on CYP337B1 expression, and possible tians-acWng effects on expression of other genes.

KW - cDNA-AFLP

KW - Cotton bollworm

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KW - Linkage mapping

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Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA-AFLP

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Keywords

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