Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry

Lucas C. Lazari; Livia Rosa-Fernandes; Giuseppe Palmisano

doi:10.1007/978-1-0716-2395-4_13

Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry

Lucas C. Lazari, Livia Rosa-Fernandes, Giuseppe Palmisano

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Matrix-assisted laser desorption/ionization source coupled with time-of-flight mass analyzer mass spectrometry (MALDI-TOF MS) is being widely used to obtain proteomic profiles for clinical purposes, as a fast, low-cost, robust, and efficient technique. Here we describe a method for biofluid analysis using MALDI-TOF MS for rapid acquisition of proteomic signatures of COVID-19 infected patients. By using solid-phase extraction, the method allows the analysis of biofluids in less than 15 min.

Original language	English
Title of host publication	Multiplex biomarker techniques
Subtitle of host publication	methods and applications for COVID-19 disease diagnosis and risk stratification
Editors	Paul C. Guest
Place of Publication	New York, NY
Publisher	Springer, Springer Nature
Chapter	13
Pages	175-182
Number of pages	8
ISBN (Electronic)	9781071623954
ISBN (Print)	9781071623947
DOIs	https://doi.org/10.1007/978-1-0716-2395-4_13
Publication status	Published - 2022
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	2511
ISSN (Print)	1064-3745

Keywords

Biomarkers
COVID-19/diagnosis
Humans
Proteomics/methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

Access to Document

10.1007/978-1-0716-2395-4_13

Cite this

Lazari, L. C., Rosa-Fernandes, L., & Palmisano, G. (2022). Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry. In P. C. Guest (Ed.), Multiplex biomarker techniques: methods and applications for COVID-19 disease diagnosis and risk stratification (pp. 175-182). (Methods in Molecular Biology; Vol. 2511). Springer, Springer Nature. https://doi.org/10.1007/978-1-0716-2395-4_13

Lazari, Lucas C. ; Rosa-Fernandes, Livia ; Palmisano, Giuseppe. / Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry. Multiplex biomarker techniques: methods and applications for COVID-19 disease diagnosis and risk stratification. editor / Paul C. Guest. New York, NY : Springer, Springer Nature, 2022. pp. 175-182 (Methods in Molecular Biology).

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abstract = "Matrix-assisted laser desorption/ionization source coupled with time-of-flight mass analyzer mass spectrometry (MALDI-TOF MS) is being widely used to obtain proteomic profiles for clinical purposes, as a fast, low-cost, robust, and efficient technique. Here we describe a method for biofluid analysis using MALDI-TOF MS for rapid acquisition of proteomic signatures of COVID-19 infected patients. By using solid-phase extraction, the method allows the analysis of biofluids in less than 15 min.",

keywords = "Biomarkers, COVID-19/diagnosis, Humans, Proteomics/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods",

author = "Lazari, {Lucas C.} and Livia Rosa-Fernandes and Giuseppe Palmisano",

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Lazari, LC, Rosa-Fernandes, L & Palmisano, G 2022, Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry. in PC Guest (ed.), Multiplex biomarker techniques: methods and applications for COVID-19 disease diagnosis and risk stratification. Methods in Molecular Biology, vol. 2511, Springer, Springer Nature, New York, NY, pp. 175-182. https://doi.org/10.1007/978-1-0716-2395-4_13

Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry. / Lazari, Lucas C.; Rosa-Fernandes, Livia; Palmisano, Giuseppe.
Multiplex biomarker techniques: methods and applications for COVID-19 disease diagnosis and risk stratification. ed. / Paul C. Guest. New York, NY: Springer, Springer Nature, 2022. p. 175-182 (Methods in Molecular Biology; Vol. 2511).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry

AU - Lazari, Lucas C.

AU - Rosa-Fernandes, Livia

AU - Palmisano, Giuseppe

PY - 2022

Y1 - 2022

N2 - Matrix-assisted laser desorption/ionization source coupled with time-of-flight mass analyzer mass spectrometry (MALDI-TOF MS) is being widely used to obtain proteomic profiles for clinical purposes, as a fast, low-cost, robust, and efficient technique. Here we describe a method for biofluid analysis using MALDI-TOF MS for rapid acquisition of proteomic signatures of COVID-19 infected patients. By using solid-phase extraction, the method allows the analysis of biofluids in less than 15 min.

AB - Matrix-assisted laser desorption/ionization source coupled with time-of-flight mass analyzer mass spectrometry (MALDI-TOF MS) is being widely used to obtain proteomic profiles for clinical purposes, as a fast, low-cost, robust, and efficient technique. Here we describe a method for biofluid analysis using MALDI-TOF MS for rapid acquisition of proteomic signatures of COVID-19 infected patients. By using solid-phase extraction, the method allows the analysis of biofluids in less than 15 min.

KW - Biomarkers

KW - COVID-19/diagnosis

KW - Humans

KW - Proteomics/methods

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

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SN - 9781071623947

T3 - Methods in Molecular Biology

SP - 175

EP - 182

BT - Multiplex biomarker techniques

A2 - Guest, Paul C.

PB - Springer, Springer Nature

CY - New York, NY

ER -

Lazari LC, Rosa-Fernandes L, Palmisano G. Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry. In Guest PC, editor, Multiplex biomarker techniques: methods and applications for COVID-19 disease diagnosis and risk stratification. New York, NY: Springer, Springer Nature. 2022. p. 175-182. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-2395-4_13

Identification of circulating biomarkers of COVID-19 using MALDI-TOF mass spectrometry

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