Identification of four IgE-reactive proteins in raspberry (Rubus ideaeus L.)

Gorji Marzban, Anita Herndl, Daniel Kolarich, Fatemeh Maghuly, Agata Mansfeld, Wolfgang Hemmer, Hermann Katinger, Margit Laimer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

IgE-reactive proteins in raspberry (Rubus ideaus L.) were identified using PCR, RT-PCR, 2-DE and MS/MS peptide sequencing. Specific polyclonal antibodies and patient sera were used in Western blotting to identify crossreactive epitopes. Initially, two potential allergens Rub i 1 and Rub i 3 were detected using PCR, showing high sequence identity to proteins in Rosaceous species like Mal d 1 and Mal d 3 from apple, Pru av 1 and Pru av 3 from cherry and Pru p 1 and Pru p 3 from peach. Furthermore, de novo identified peptides of a protein band at about 30 kDa reacting with most of the patient sera tested (>80%) revealed a high sequence homology with class III chitinases. Raspberry chitinase, when subjected to glycoproteomic analysis, showed typical complex plant-type N-glycans with a core α1,3 fucose and a β1,2 xylose at least at one position, indicating the presence of cross-reacting carbohydrate determinants (CCDs). Finally, MS/MS analysis revealed an IgE-reactive raspberry cyclophilin, homologous to Bet v 7. Results obtained suggest that the consumption of raspberries might be responsible for adverse reactions in sensitised individuals.

Original languageEnglish
Pages (from-to)1497-1506
Number of pages10
JournalMolecular Nutrition and Food Research
Volume52
Issue number12
DOIs
Publication statusPublished - Dec 2008

Keywords

  • Class III chitinase
  • Cyclophilin
  • Rub i 1
  • Rub i 3
  • Small fruits

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