TY - JOUR
T1 - Identification of gut bacterial community and their effect on the fecundity of pumpkin fly, Bactrocera tau (Walker)
AU - Khan, M.
AU - Mahin, A. A.
AU - Pramanik, M. K.
AU - Akter, H.
PY - 2014
Y1 - 2014
N2 - The bacteria in fruit flies alimentary tract have been known in advantages in their biology of the insect hosts. Different biochemical, gram reaction and motility tests was performed to identify the mid-gut bacterial community of laboratory host reared pumpkin fly, Bactrocera tau (Walker) (Diptera:Tephritidae). Colony characterization of the isolated bacteria was conducted on nutrient agar and MacConey agar plates. Isolated gut-bacterial species viz., Proteus rettgeri and Klebsiella oxytoca was examined by incorporated with protein (casein:yeast extract:sugar, 1:1:2) and sugar diet to study the effect of bacteria supplemented &ets on the ovariole number and egg production of B. tau. Eight genera and nine bacterial species were identified under the family Enterobacteriaceae. The identified bacterial genera were Proteus, Klebsiella, Streptobacillus, Alcaligenes, Haemophilus, Erwinia, Chromobacterium and Flauobacterium. The mean ovariole number per ovary was recorded as 20.66±2.51, 20.56±3.53 and 22.41±3.75 for B. tau fed on P. rettgeri, K. oqtocaincorporated protein &ets and only protein &et, respectively. Experimental result revealed no significant influence of gut bacteria added adult &ets on egglfemalelday of B. tau fed on above mentioned diet treatments.
AB - The bacteria in fruit flies alimentary tract have been known in advantages in their biology of the insect hosts. Different biochemical, gram reaction and motility tests was performed to identify the mid-gut bacterial community of laboratory host reared pumpkin fly, Bactrocera tau (Walker) (Diptera:Tephritidae). Colony characterization of the isolated bacteria was conducted on nutrient agar and MacConey agar plates. Isolated gut-bacterial species viz., Proteus rettgeri and Klebsiella oxytoca was examined by incorporated with protein (casein:yeast extract:sugar, 1:1:2) and sugar diet to study the effect of bacteria supplemented &ets on the ovariole number and egg production of B. tau. Eight genera and nine bacterial species were identified under the family Enterobacteriaceae. The identified bacterial genera were Proteus, Klebsiella, Streptobacillus, Alcaligenes, Haemophilus, Erwinia, Chromobacterium and Flauobacterium. The mean ovariole number per ovary was recorded as 20.66±2.51, 20.56±3.53 and 22.41±3.75 for B. tau fed on P. rettgeri, K. oqtocaincorporated protein &ets and only protein &et, respectively. Experimental result revealed no significant influence of gut bacteria added adult &ets on egglfemalelday of B. tau fed on above mentioned diet treatments.
KW - Bactrocera tau
KW - gut bacteria
KW - probiotic
KW - ovariole number
KW - fecundity
UR - http://www.scopus.com/inward/record.url?scp=84896839831&partnerID=8YFLogxK
U2 - 10.3923/je.2014.68.77
DO - 10.3923/je.2014.68.77
M3 - Article
AN - SCOPUS:84896839831
SN - 1812-5670
VL - 11
SP - 68
EP - 77
JO - Journal of Entomology
JF - Journal of Entomology
IS - 2
ER -