Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37

Barbara J Kelly, Eve Diefenbach, Cornel Fraefel, Russell J Diefenbach

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The herpes simplex virus type 1 (HSV-1) structural tegument protein pUL37, which is conserved across the Herpesviridae family, is known to be essential for secondary envelopment during the egress of viral particles. To shed light on additional roles of pUL37 during viral replication a yeast two-hybrid screen of a human brain cDNA library was undertaken. This screen identified ten host cell proteins as potential pUL37 interactors. One of the interactors, serine threonine kinase TAOK3, was subsequently confirmed to interact with pUL37 using an in vitro pulldown assay. Such host cell/pUL37 interactions provide further insights into the multifunctional role of this herpesviral tegument protein.

Original languageEnglish
Pages (from-to)961-965
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume417
Issue number3
DOIs
Publication statusPublished - 20 Jan 2012
Externally publishedYes

Keywords

  • Brain
  • Gene Library
  • Herpes Simplex
  • Herpesvirus 1, Human
  • Host-Pathogen Interactions
  • Humans
  • Protein-Serine-Threonine Kinases
  • Two-Hybrid System Techniques
  • Viral Structural Proteins
  • Virus Replication
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fingerprint Dive into the research topics of 'Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37'. Together they form a unique fingerprint.

Cite this