Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37

Barbara J Kelly; Eve Diefenbach; Cornel Fraefel; Russell J Diefenbach

doi:10.1016/j.bbrc.2011.12.044

Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37

Barbara J Kelly, Eve Diefenbach, Cornel Fraefel, Russell J Diefenbach

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The herpes simplex virus type 1 (HSV-1) structural tegument protein pUL37, which is conserved across the Herpesviridae family, is known to be essential for secondary envelopment during the egress of viral particles. To shed light on additional roles of pUL37 during viral replication a yeast two-hybrid screen of a human brain cDNA library was undertaken. This screen identified ten host cell proteins as potential pUL37 interactors. One of the interactors, serine threonine kinase TAOK3, was subsequently confirmed to interact with pUL37 using an in vitro pulldown assay. Such host cell/pUL37 interactions provide further insights into the multifunctional role of this herpesviral tegument protein.

Original language	English
Pages (from-to)	961-965
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	417
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.12.044
Publication status	Published - 20 Jan 2012
Externally published	Yes

Keywords

Brain
Gene Library
Herpes Simplex
Herpesvirus 1, Human
Host-Pathogen Interactions
Humans
Protein-Serine-Threonine Kinases
Two-Hybrid System Techniques
Viral Structural Proteins
Virus Replication
Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.bbrc.2011.12.044

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title = "Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37",

abstract = "The herpes simplex virus type 1 (HSV-1) structural tegument protein pUL37, which is conserved across the Herpesviridae family, is known to be essential for secondary envelopment during the egress of viral particles. To shed light on additional roles of pUL37 during viral replication a yeast two-hybrid screen of a human brain cDNA library was undertaken. This screen identified ten host cell proteins as potential pUL37 interactors. One of the interactors, serine threonine kinase TAOK3, was subsequently confirmed to interact with pUL37 using an in vitro pulldown assay. Such host cell/pUL37 interactions provide further insights into the multifunctional role of this herpesviral tegument protein.",

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author = "Kelly, {Barbara J} and Eve Diefenbach and Cornel Fraefel and Diefenbach, {Russell J}",

year = "2012",

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doi = "10.1016/j.bbrc.2011.12.044",

language = "English",

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AU - Kelly, Barbara J

AU - Diefenbach, Eve

AU - Fraefel, Cornel

AU - Diefenbach, Russell J

PY - 2012/1/20

Y1 - 2012/1/20

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AB - The herpes simplex virus type 1 (HSV-1) structural tegument protein pUL37, which is conserved across the Herpesviridae family, is known to be essential for secondary envelopment during the egress of viral particles. To shed light on additional roles of pUL37 during viral replication a yeast two-hybrid screen of a human brain cDNA library was undertaken. This screen identified ten host cell proteins as potential pUL37 interactors. One of the interactors, serine threonine kinase TAOK3, was subsequently confirmed to interact with pUL37 using an in vitro pulldown assay. Such host cell/pUL37 interactions provide further insights into the multifunctional role of this herpesviral tegument protein.

KW - Brain

KW - Gene Library

KW - Herpes Simplex

KW - Herpesvirus 1, Human

KW - Host-Pathogen Interactions

KW - Humans

KW - Protein-Serine-Threonine Kinases

KW - Two-Hybrid System Techniques

KW - Viral Structural Proteins

KW - Virus Replication

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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DO - 10.1016/j.bbrc.2011.12.044

M3 - Article

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VL - 417

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Identification of host cell proteins which interact with herpes simplex virus type 1 tegument protein pUL37

Abstract

Keywords

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