Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry

Jennifer T. Saville, Zhenjun Zhao, Mark D P Willcox, Manjula A. Ariyavidana, Stephen J. Blanksby*, Todd W. Mitchell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Meibum is believed to be the major source of tear film lipids, which are vital in the prevention of excess evaporation of the aqueous phase. The complete lipid composition of meibum has yet to be established. While earlier studies reported the presence of phospholipids in human meibum, recent mass spectrometric studies have not detected them. In this study we use electrospray ionisation tandem mass spectrometry to investigate the presence of phospholipids in meibum and provide comparison to the phospholipid profile of tears.Lipids were extracted from human meibum and tear samples using standard biphasic methods and analysed by nano-electrospray ionisation tandem mass spectrometry using targeted ion scans. A total of 35 choline-containing phospholipids were identified in meibum and the profile of these was similar to that observed in tears, suggesting tear lipids are derived from meibum. The results shown here highlight the need for a combination of optimised techniques to enable the identification of the large range of lipid classes in meibum.

Original languageEnglish
Pages (from-to)238-240
Number of pages3
JournalExperimental Eye Research
Volume92
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

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