Identification of spinally projecting neurons in the rostral ventrolateral medulla in vivo

Simon McMullan*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


    Putative sympathetic premotor neurons in the rostral ventrolateral medulla are critically important in the regulation of sympathetic vasomotor tone and are responsible for mediating many cardiovascular reflexes. In the rat, these neurons lie within a small area of the brainstem immediately caudal to the facial nucleus and can be distinguished from neighbouring cells by their axonal projections to the thoracic spinal cord, where they are thought to form synapses with sympathetic preganglionic neurons. This protocol describes the steps required for identification of sympathetic premotor neurons in acute experiments in vivo. It provides a detailed description of the methodology we use routinely to electrophysiologically map the topography of the facial nucleus and an account of the steps needed to conduct the antidromic collision test.

    Original languageEnglish
    Title of host publicationStimulation and Inhibition of Neurons
    EditorsPaul M. Pilowsky, Melissa M.J. Farnham, Angelina Y. Fong
    Place of PublicationNew York
    PublisherHumana Press
    Number of pages18
    ISBN (Electronic)9781627032339
    ISBN (Print)9781627032322, 40021988138
    Publication statusPublished - 2013

    Publication series

    PublisherHumana Press
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045


    Dive into the research topics of 'Identification of spinally projecting neurons in the rostral ventrolateral medulla in vivo'. Together they form a unique fingerprint.

    Cite this