Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer

Ferdous Rastgar Jazii*, Zahra Najafi, Reza Malekzadeh, Thomas P. Conrads, Abed Ali Ziaee, Christian Abnet, Mansour Yazdznbod, Ali Asghar Karkhane, Ghasem H. Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

118 Citations (Scopus)

Abstract

Aim: To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus (SCCE) in Iranian patients and compare our results with former reports by using proteomics. Methods: Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed. Results: Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin (TMβ), myosin light chain 2 (and its isoform), myosin regulatory light chain 2, peroxyredoxin 2, annexin I and an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 (HSP70), TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratin I, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ, HSP70, annexin I, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers. Conclusion: Our study not only supports the involvement of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ.

Original languageEnglish
Pages (from-to)7104-7112
Number of pages9
JournalWorld Journal of Gastroenterology
Volume12
Issue number44
DOIs
Publication statusPublished - 28 Nov 2006
Externally publishedYes

Keywords

  • Esophageal
  • Esophagus
  • Polypeptide marker
  • Proteomics
  • Squamous cell carcinoma
  • Two dimensional electrophoresis

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