Identification of T-cell epitopes adjacent to neutralizing antigenic domains on the fusion protein of respiratory syncytial virus

C. Corvaisier, G. Guillemin, C. Bourgeois, J. B. Bour, E. Kohli, P. Pothier*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

F glycoprotein has been identified as an important target structure in the immunological response following respiratory syncytial virus (RSV) infection. Two sequential B epitopes corresponding to amino acids 200 to 225 and 255 to 278 have already been defined with anti-RSV rabbit serum. The T helper response to peptides which belong to these sequences was investigated in this study. Proliferative T-cell responses to these peptides were analysed in BALB/c mice (H-2d) and others strains: SJL (H-2s), C3H/He (H-2k), B10.BR (H-2k) and C57BL/6 (H-2b). By using various strategies, two T-cell epitopes were identified in the amino acid 200 to 225 and 255 to 278 regions, close to a neutralizing epitope. The T-cell responses to these peptides were H-2- restricted. In addition, the peptide 255-278 was able to stimulate T cells that responded to a subsequent immunization with F glycoprotein in vitro.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalResearch in Virology
Volume144
Issue numberC
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • Identification
  • Respiratory syncytical virus, F protein, T-cell epitope, Cellular immunity

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