TY - JOUR
T1 - Identification of T-cell epitopes adjacent to neutralizing antigenic domains on the fusion protein of respiratory syncytial virus
AU - Corvaisier, C.
AU - Guillemin, G.
AU - Bourgeois, C.
AU - Bour, J. B.
AU - Kohli, E.
AU - Pothier, P.
PY - 1993
Y1 - 1993
N2 - F glycoprotein has been identified as an important target structure in the immunological response following respiratory syncytial virus (RSV) infection. Two sequential B epitopes corresponding to amino acids 200 to 225 and 255 to 278 have already been defined with anti-RSV rabbit serum. The T helper response to peptides which belong to these sequences was investigated in this study. Proliferative T-cell responses to these peptides were analysed in BALB/c mice (H-2d) and others strains: SJL (H-2s), C3H/He (H-2k), B10.BR (H-2k) and C57BL/6 (H-2b). By using various strategies, two T-cell epitopes were identified in the amino acid 200 to 225 and 255 to 278 regions, close to a neutralizing epitope. The T-cell responses to these peptides were H-2- restricted. In addition, the peptide 255-278 was able to stimulate T cells that responded to a subsequent immunization with F glycoprotein in vitro.
AB - F glycoprotein has been identified as an important target structure in the immunological response following respiratory syncytial virus (RSV) infection. Two sequential B epitopes corresponding to amino acids 200 to 225 and 255 to 278 have already been defined with anti-RSV rabbit serum. The T helper response to peptides which belong to these sequences was investigated in this study. Proliferative T-cell responses to these peptides were analysed in BALB/c mice (H-2d) and others strains: SJL (H-2s), C3H/He (H-2k), B10.BR (H-2k) and C57BL/6 (H-2b). By using various strategies, two T-cell epitopes were identified in the amino acid 200 to 225 and 255 to 278 regions, close to a neutralizing epitope. The T-cell responses to these peptides were H-2- restricted. In addition, the peptide 255-278 was able to stimulate T cells that responded to a subsequent immunization with F glycoprotein in vitro.
KW - Identification
KW - Respiratory syncytical virus, F protein, T-cell epitope, Cellular immunity
UR - http://www.scopus.com/inward/record.url?scp=0027199970&partnerID=8YFLogxK
U2 - 10.1016/S0923-2516(06)80022-1
DO - 10.1016/S0923-2516(06)80022-1
M3 - Article
C2 - 7685537
AN - SCOPUS:0027199970
SN - 0923-2516
VL - 144
SP - 141
EP - 150
JO - Research in Virology
JF - Research in Virology
IS - C
ER -