Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

Andy C. M. Chang; Martin B. Slade; Keith L. Williams

doi:10.1016/0147-619X(90)90004-V

Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

Andy C. M. Chang, Martin B. Slade, Keith L. Williams

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts intrans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T · A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.

Original language	English
Pages (from-to)	208-217
Number of pages	10
Journal	Plasmid
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/0147-619X(90)90004-V
Publication status	Published - 1990

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title = "Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2",

abstract = "Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts intrans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp {"}TGTCATGACA{"} palindromes separated by a poly(T · A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.",

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T1 - Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

AU - Chang, Andy C. M.

AU - Slade, Martin B.

AU - Williams, Keith L.

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N2 - Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts intrans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T · A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.

AB - Ddp2 is a 5.8-kb, high-copy-number, nuclear plasmid found in the eukaryote Dictyostelium discoideum. We have identified two functional domains, a large open reading frame (Rep gene) and a 626-bp fragment containing an origin of replication (ori). The ori, when cloned into a shuttle vector, confers stable extrachromosomal replication in D. discoideum, provided that the Rep gene, which acts intrans, is integrated into the host genome. Ddp2 carries a 501-bp imperfect inverted repeat, and part of the ori overlaps with one of these repeats. The ori sequence contains two direct repeats of 49 bp comprising two 10-bp "TGTCATGACA" palindromes separated by a poly(T · A) sequence. Deletion of either 49-bp repeat abolished extrachromosomal replication.

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Identification of the origin of replication of the eukaryote Dictyostelium discoideum nuclear plasmid Ddp2

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