Identification of wallaby milk whey proteins separated by two-dimensional electrophoresis, using amino acid analysis and sequence tagging

Mark P. Molloy, Ben R. Herbert, Jun X. Yan, Keith L. Williams*, Andrew A. Gooley

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Micropreparative two-dimensional polyacrylamide gel electrophoresis has been used to separate milk whey proteins from the Tammar wallaby (Macropus eugenii). We have used a combination of amino acid analysis and N-terminal sequence tagging as a rapid and sensitive method to identify the major whey proteins. Using these techniques, we confidently identified α-lactalbumin and late lactation protein. While these are the only two M. eugenii whey proteins with a corresponding SWISS-PROT entry, we demonstrate that by using amino acid analysis and matching across species boundaries, we can identify previously unsequenced conserved wallaby whey proteins including β-lactoglobulin and serum albumin.

Original languageEnglish
Pages (from-to)1073-1078
Number of pages6
JournalElectrophoresis
Volume18
Issue number7
DOIs
Publication statusPublished - Jun 1997

Keywords

  • amino acid analysis
  • protein identification
  • sequence tagging
  • two-dimensional polyacrylamide gele electrophoresis
  • whey proteins

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