Identification of zoonotic Giardia genotypes in marsupials in Australia

Jacqui Thompson, Rongchang Yang, Michelle Power, Jasmin Hufschmid, Ian Beveridge, Simon Reid, Josephine Ng, Anthony Armson, Una Ryan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A total of 421 fecal samples from a variety of captive and wild marsupial hosts in Western Australia, Victoria and South Australia were screened for the presence of Giardia species/genotypes using PCR and sequence analysis of a fragment of the 18S rRNA gene. Giardia spp. were identified in 13.4% (28/209) of samples from captive marsupials and 13.7% (29/212) of samples from wild marsupials. Sequence analysis at the 18S locus identified the zoonotic Giardia duodenalis Genotypes A and B in both captive and wild marsupials. Eight isolates were typed as genotype B3 and B4 at the gdh locus, although 7/8 were typed as genotype A at the 18S rRNA locus. The possible reasons for this discordance are discussed. This is the first report of genotype B and only the second report of genotype A in marsupials. As some of the genotype B isolates were identical to human-derived Giardia gdh sequences, these results suggest that marsupials in catchments may pose a public health risk and therefore warrant further investigation.

LanguageEnglish
Pages88-93
Number of pages6
JournalExperimental Parasitology
Volume120
Issue number1
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Giardia
Marsupialia
Zoonoses
Genotype
Sequence Analysis
Giardia lamblia
Western Australia
South Australia
Victoria
rRNA Genes
Public Health
Polymerase Chain Reaction

Cite this

Thompson, J., Yang, R., Power, M., Hufschmid, J., Beveridge, I., Reid, S., ... Ryan, U. (2008). Identification of zoonotic Giardia genotypes in marsupials in Australia. Experimental Parasitology, 120(1), 88-93. https://doi.org/10.1016/j.exppara.2008.05.002
Thompson, Jacqui ; Yang, Rongchang ; Power, Michelle ; Hufschmid, Jasmin ; Beveridge, Ian ; Reid, Simon ; Ng, Josephine ; Armson, Anthony ; Ryan, Una. / Identification of zoonotic Giardia genotypes in marsupials in Australia. In: Experimental Parasitology. 2008 ; Vol. 120, No. 1. pp. 88-93.
@article{e8848485812b46a78bb9420b223c4a52,
title = "Identification of zoonotic Giardia genotypes in marsupials in Australia",
abstract = "A total of 421 fecal samples from a variety of captive and wild marsupial hosts in Western Australia, Victoria and South Australia were screened for the presence of Giardia species/genotypes using PCR and sequence analysis of a fragment of the 18S rRNA gene. Giardia spp. were identified in 13.4{\%} (28/209) of samples from captive marsupials and 13.7{\%} (29/212) of samples from wild marsupials. Sequence analysis at the 18S locus identified the zoonotic Giardia duodenalis Genotypes A and B in both captive and wild marsupials. Eight isolates were typed as genotype B3 and B4 at the gdh locus, although 7/8 were typed as genotype A at the 18S rRNA locus. The possible reasons for this discordance are discussed. This is the first report of genotype B and only the second report of genotype A in marsupials. As some of the genotype B isolates were identical to human-derived Giardia gdh sequences, these results suggest that marsupials in catchments may pose a public health risk and therefore warrant further investigation.",
author = "Jacqui Thompson and Rongchang Yang and Michelle Power and Jasmin Hufschmid and Ian Beveridge and Simon Reid and Josephine Ng and Anthony Armson and Una Ryan",
year = "2008",
month = "9",
doi = "10.1016/j.exppara.2008.05.002",
language = "English",
volume = "120",
pages = "88--93",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "1",

}

Thompson, J, Yang, R, Power, M, Hufschmid, J, Beveridge, I, Reid, S, Ng, J, Armson, A & Ryan, U 2008, 'Identification of zoonotic Giardia genotypes in marsupials in Australia', Experimental Parasitology, vol. 120, no. 1, pp. 88-93. https://doi.org/10.1016/j.exppara.2008.05.002

Identification of zoonotic Giardia genotypes in marsupials in Australia. / Thompson, Jacqui; Yang, Rongchang; Power, Michelle; Hufschmid, Jasmin; Beveridge, Ian; Reid, Simon; Ng, Josephine; Armson, Anthony; Ryan, Una.

In: Experimental Parasitology, Vol. 120, No. 1, 09.2008, p. 88-93.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of zoonotic Giardia genotypes in marsupials in Australia

AU - Thompson, Jacqui

AU - Yang, Rongchang

AU - Power, Michelle

AU - Hufschmid, Jasmin

AU - Beveridge, Ian

AU - Reid, Simon

AU - Ng, Josephine

AU - Armson, Anthony

AU - Ryan, Una

PY - 2008/9

Y1 - 2008/9

N2 - A total of 421 fecal samples from a variety of captive and wild marsupial hosts in Western Australia, Victoria and South Australia were screened for the presence of Giardia species/genotypes using PCR and sequence analysis of a fragment of the 18S rRNA gene. Giardia spp. were identified in 13.4% (28/209) of samples from captive marsupials and 13.7% (29/212) of samples from wild marsupials. Sequence analysis at the 18S locus identified the zoonotic Giardia duodenalis Genotypes A and B in both captive and wild marsupials. Eight isolates were typed as genotype B3 and B4 at the gdh locus, although 7/8 were typed as genotype A at the 18S rRNA locus. The possible reasons for this discordance are discussed. This is the first report of genotype B and only the second report of genotype A in marsupials. As some of the genotype B isolates were identical to human-derived Giardia gdh sequences, these results suggest that marsupials in catchments may pose a public health risk and therefore warrant further investigation.

AB - A total of 421 fecal samples from a variety of captive and wild marsupial hosts in Western Australia, Victoria and South Australia were screened for the presence of Giardia species/genotypes using PCR and sequence analysis of a fragment of the 18S rRNA gene. Giardia spp. were identified in 13.4% (28/209) of samples from captive marsupials and 13.7% (29/212) of samples from wild marsupials. Sequence analysis at the 18S locus identified the zoonotic Giardia duodenalis Genotypes A and B in both captive and wild marsupials. Eight isolates were typed as genotype B3 and B4 at the gdh locus, although 7/8 were typed as genotype A at the 18S rRNA locus. The possible reasons for this discordance are discussed. This is the first report of genotype B and only the second report of genotype A in marsupials. As some of the genotype B isolates were identical to human-derived Giardia gdh sequences, these results suggest that marsupials in catchments may pose a public health risk and therefore warrant further investigation.

UR - http://www.scopus.com/inward/record.url?scp=48649109142&partnerID=8YFLogxK

U2 - 10.1016/j.exppara.2008.05.002

DO - 10.1016/j.exppara.2008.05.002

M3 - Article

VL - 120

SP - 88

EP - 93

JO - Experimental Parasitology

T2 - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 1

ER -