Identification of zoonotic Giardia genotypes in marsupials in Australia

Jacqui Thompson, Rongchang Yang, Michelle Power, Jasmin Hufschmid, Ian Beveridge, Simon Reid, Josephine Ng, Anthony Armson, Una Ryan*

*Corresponding author for this work

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A total of 421 fecal samples from a variety of captive and wild marsupial hosts in Western Australia, Victoria and South Australia were screened for the presence of Giardia species/genotypes using PCR and sequence analysis of a fragment of the 18S rRNA gene. Giardia spp. were identified in 13.4% (28/209) of samples from captive marsupials and 13.7% (29/212) of samples from wild marsupials. Sequence analysis at the 18S locus identified the zoonotic Giardia duodenalis Genotypes A and B in both captive and wild marsupials. Eight isolates were typed as genotype B3 and B4 at the gdh locus, although 7/8 were typed as genotype A at the 18S rRNA locus. The possible reasons for this discordance are discussed. This is the first report of genotype B and only the second report of genotype A in marsupials. As some of the genotype B isolates were identical to human-derived Giardia gdh sequences, these results suggest that marsupials in catchments may pose a public health risk and therefore warrant further investigation.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalExperimental Parasitology
Volume120
Issue number1
DOIs
Publication statusPublished - Sep 2008

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