Identity and distribution of American foulbrood (Paenibacillus larvae) in South Africa

Yordan V. Hristov, Johannes J. Le Roux, Michael H. Allsopp, Theresa C. Wossler*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    Abstract

    American foulbrood (AFB) is a serious and near-global disease of honey bees and was recently discovered in South Africa. The causative pathogen, Paenibacillus larvae, comprises various strains. It is currently unknown which of these are present in South Africa and what their distribution is. We address this issue by genotyping P. larvae isolates using repetitive element fingerprinting (rep-PCR) markers and microfabricated chip technology. A total of 71 brood, honey and wax samples were collected opportunistically in South Africa. Twenty-three samples tested positive for P. larvae (32.4%). Diagnostic rep-PCR fingerprinting using ERIC, BOX A1R and MBO REP1 primers found all P. larvae isolates in South Africa to correspond to the ERIC I genotype, with two subtypes revealed, Ab (13 samples) and ab (10 samples). These results are in agreement with the global prevalence of the ERIC I AFB genotype among P. larvae-infected honey bee colonies.

    Original languageEnglish
    Number of pages8
    JournalJournal of Apicultural Research
    Early online date10 Mar 2021
    DOIs
    Publication statusE-pub ahead of print - 10 Mar 2021

    Bibliographical note

    Funding Information:
    Funding for this work was obtained from the National Research Foundation (separate grants awarded to TCW and JJLR). Many thanks to the South African Department of Agriculture, Forestry and Fisheries. Special thanks to Ms. Megan Mathese for her help with analysis of the samples.

    Publisher Copyright:
    © 2021 International Bee Research Association.

    Copyright:
    Copyright 2021 Elsevier B.V., All rights reserved.

    Keywords

    • American foulbrood
    • honey bees
    • LabChip technology
    • Paenibacillus larvae
    • rep-PCR
    • South Africa

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