TY - JOUR
T1 - In vitro biosynthesis of androgens in the Australian lungfish, Neoceratodus forsteri
AU - Joss, Jean M. P.
AU - Edwards, Ashley
AU - Kime, David E.
PY - 1996/3
Y1 - 1996/3
N2 - The serum concentration of testosterone was estimated from a population of wild lungfish over 6-7 years of sampling. Male lungfish were found to have high circulating levels of testosterone (~50 ng/ml) which varied seasonally and could be correlated with spermatogenesis as judged by testis histology. Incubation of testis tissue slices with [3H]progesterone, [3H]17- hydroxyprogesterone, or [3H]testosterone confirmed that testosterone is the major androgen in Neoceratodus. Not even trace amounts of 11-keto- or 11β- hydroxytestosterone or 5α-dihydrotestosterone could be identified by TLC separations. There was little or no conjugation of steroids by the testes, except during the spawning season, when glucuronides of androstenedione and testosterone were produced.
AB - The serum concentration of testosterone was estimated from a population of wild lungfish over 6-7 years of sampling. Male lungfish were found to have high circulating levels of testosterone (~50 ng/ml) which varied seasonally and could be correlated with spermatogenesis as judged by testis histology. Incubation of testis tissue slices with [3H]progesterone, [3H]17- hydroxyprogesterone, or [3H]testosterone confirmed that testosterone is the major androgen in Neoceratodus. Not even trace amounts of 11-keto- or 11β- hydroxytestosterone or 5α-dihydrotestosterone could be identified by TLC separations. There was little or no conjugation of steroids by the testes, except during the spawning season, when glucuronides of androstenedione and testosterone were produced.
UR - http://www.scopus.com/inward/record.url?scp=0029878825&partnerID=8YFLogxK
U2 - 10.1006/gcen.1996.0028
DO - 10.1006/gcen.1996.0028
M3 - Article
C2 - 8729935
AN - SCOPUS:0029878825
SN - 0016-6480
VL - 101
SP - 256
EP - 263
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 3
ER -