In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles

Alfonso E. Garcia-Bennett*, Niclas König, Ninnie Abrahamsson, Mariya Kozhevnikova, Chunfang Zhou, Carl Trolle, Stanislava Pankratova, Vladimir Berezin, Elena N. Kozlova

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica.

Original languageEnglish
Pages (from-to)2457-2466
Number of pages10
JournalNanomedicine
Volume9
Issue number16
DOIs
Publication statusPublished - 1 Nov 2014
Externally publishedYes

Keywords

  • cell transplant
  • differentiation
  • mesoporous material
  • nanomedicine
  • stem cell

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