Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models

Jóyce dos Santos Lencina; Iluska Senna Bonfá Moslaves; Jéssica de Araujo Isaias Muller; Raquel Carvalho; Carolina Amianti; Izadora Bonfim; Flávio Macedo Alves; Carlos Alexandre Carollo; Luciane Candeloro; Antonio Alves dos Santos Júnior; Denise Brentan da Silva; Mônica Cristina Toffoli Kadri

doi:10.1016/j.jep.2021.114461

Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models

Jóyce dos Santos Lencina, Iluska Senna Bonfá Moslaves, Jéssica de Araujo Isaias Muller, Raquel Carvalho, Carolina Amianti, Izadora Bonfim, Flávio Macedo Alves, Carlos Alexandre Carollo, Luciane Candeloro, Antonio Alves dos Santos Júnior, Denise Brentan da Silva, Mônica Cristina Toffoli Kadri^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Lantana canescens is popularly known in Brazil as “cidreirinha” or “chumbinho-branco”. It is found in Pantanal biome and its flowers and leaves are used in traditional medicine to treat pain and inflammation. Information about this species is limited to the activity of isolated essential oils. Studies with different extracts, composition, and biological properties are still scarce.

Aim of this study: The objective of this study was to evaluate the anti-inflammatory and anti-hyperalgesic activity of the hydroethanolic extract of L. canescens aerial parts.

Materials and methods: The hydroethanolic extract L. canescens aerial parts (HELc) was analyzed using HPLC-DAD-EM. Male and female Swiss mice weighing 18–25 g were used in the in vivo assays. Acute toxicity was assessed (2000 mg/kg); anti-inflammatory activity through paw edema, mast cell degranulation and peritonitis, and anti-hyperalgesic activity through abdominal writhing assays induced by acetic acid and formalin sensitization, were evaluated using the doses of 3, 30 and 300 mg/kg.

Results: The phytochemical characterization of HELc confirmed the presence of glycosylated iridoids (theveside, theviridoside), verbascosides and flavonoids. The HELc did not present toxicity in the evaluated dose. HELc reduced formation of paw edema, degranulation of peritoneal mast cells and infiltration of polymorphonuclear cells into the animals peritoneal cavity. In addition, HELc decreased the number of abdominal writhing induced by acetic acid and the time of paw licking in the evaluation of formalin sensitization.

Conclusions: These results confirm the anti-inflammatory and anti-hyperalgesic effects of hydroethanolic extract of L. canescens, validating the use of this plant in folk medicine.

Original language	English
Article number	114461
Pages (from-to)	1-10
Number of pages	10
Journal	Journal of Ethnopharmacology
Volume	280
Early online date	29 Jul 2021
DOIs	https://doi.org/10.1016/j.jep.2021.114461
Publication status	Published - Nov 2021
Externally published	Yes

Keywords

Medicinal plants
Preclinical assay
Pain
Inflammation
Toxicity

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10.1016/j.jep.2021.114461

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Lencina, J. D. S., Moslaves, I. S. B., Muller, J. D. A. I., Carvalho, R., Amianti, C., Bonfim, I., Alves, F. M., Carollo, C. A., Candeloro, L., Júnior, A. A. D. S., Silva, D. B. D., & Kadri, M. C. T. (2021). Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models. Journal of Ethnopharmacology, 280, 1-10. Article 114461. https://doi.org/10.1016/j.jep.2021.114461

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title = "Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models",

abstract = "Ethnopharmacological relevance: Lantana canescens is popularly known in Brazil as “cidreirinha” or “chumbinho-branco”. It is found in Pantanal biome and its flowers and leaves are used in traditional medicine to treat pain and inflammation. Information about this species is limited to the activity of isolated essential oils. Studies with different extracts, composition, and biological properties are still scarce. Aim of this study: The objective of this study was to evaluate the anti-inflammatory and anti-hyperalgesic activity of the hydroethanolic extract of L. canescens aerial parts. Materials and methods: The hydroethanolic extract L. canescens aerial parts (HELc) was analyzed using HPLC-DAD-EM. Male and female Swiss mice weighing 18–25 g were used in the in vivo assays. Acute toxicity was assessed (2000 mg/kg); anti-inflammatory activity through paw edema, mast cell degranulation and peritonitis, and anti-hyperalgesic activity through abdominal writhing assays induced by acetic acid and formalin sensitization, were evaluated using the doses of 3, 30 and 300 mg/kg. Results: The phytochemical characterization of HELc confirmed the presence of glycosylated iridoids (theveside, theviridoside), verbascosides and flavonoids. The HELc did not present toxicity in the evaluated dose. HELc reduced formation of paw edema, degranulation of peritoneal mast cells and infiltration of polymorphonuclear cells into the animals peritoneal cavity. In addition, HELc decreased the number of abdominal writhing induced by acetic acid and the time of paw licking in the evaluation of formalin sensitization. Conclusions: These results confirm the anti-inflammatory and anti-hyperalgesic effects of hydroethanolic extract of L. canescens, validating the use of this plant in folk medicine.",

keywords = "Medicinal plants, Preclinical assay, Pain, Inflammation, Toxicity",

author = "Lencina, \{J{\'o}yce dos Santos\} and Moslaves, \{Iluska Senna Bonf{\'a}\} and Muller, \{J{\'e}ssica de Araujo Isaias\} and Raquel Carvalho and Carolina Amianti and Izadora Bonfim and Alves, \{Fl{\'a}vio Macedo\} and Carollo, \{Carlos Alexandre\} and Luciane Candeloro and J{\'u}nior, \{Antonio Alves dos Santos\} and Silva, \{Denise Brentan da\} and Kadri, \{M{\^o}nica Cristina Toffoli\}",

year = "2021",

month = nov,

doi = "10.1016/j.jep.2021.114461",

language = "English",

volume = "280",

pages = "1--10",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier",

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TY - JOUR

T1 - Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models

AU - Lencina, Jóyce dos Santos

AU - Moslaves, Iluska Senna Bonfá

AU - Muller, Jéssica de Araujo Isaias

AU - Carvalho, Raquel

AU - Amianti, Carolina

AU - Bonfim, Izadora

AU - Alves, Flávio Macedo

AU - Carollo, Carlos Alexandre

AU - Candeloro, Luciane

AU - Júnior, Antonio Alves dos Santos

AU - Silva, Denise Brentan da

AU - Kadri, Mônica Cristina Toffoli

PY - 2021/11

Y1 - 2021/11

N2 - Ethnopharmacological relevance: Lantana canescens is popularly known in Brazil as “cidreirinha” or “chumbinho-branco”. It is found in Pantanal biome and its flowers and leaves are used in traditional medicine to treat pain and inflammation. Information about this species is limited to the activity of isolated essential oils. Studies with different extracts, composition, and biological properties are still scarce. Aim of this study: The objective of this study was to evaluate the anti-inflammatory and anti-hyperalgesic activity of the hydroethanolic extract of L. canescens aerial parts. Materials and methods: The hydroethanolic extract L. canescens aerial parts (HELc) was analyzed using HPLC-DAD-EM. Male and female Swiss mice weighing 18–25 g were used in the in vivo assays. Acute toxicity was assessed (2000 mg/kg); anti-inflammatory activity through paw edema, mast cell degranulation and peritonitis, and anti-hyperalgesic activity through abdominal writhing assays induced by acetic acid and formalin sensitization, were evaluated using the doses of 3, 30 and 300 mg/kg. Results: The phytochemical characterization of HELc confirmed the presence of glycosylated iridoids (theveside, theviridoside), verbascosides and flavonoids. The HELc did not present toxicity in the evaluated dose. HELc reduced formation of paw edema, degranulation of peritoneal mast cells and infiltration of polymorphonuclear cells into the animals peritoneal cavity. In addition, HELc decreased the number of abdominal writhing induced by acetic acid and the time of paw licking in the evaluation of formalin sensitization. Conclusions: These results confirm the anti-inflammatory and anti-hyperalgesic effects of hydroethanolic extract of L. canescens, validating the use of this plant in folk medicine.

AB - Ethnopharmacological relevance: Lantana canescens is popularly known in Brazil as “cidreirinha” or “chumbinho-branco”. It is found in Pantanal biome and its flowers and leaves are used in traditional medicine to treat pain and inflammation. Information about this species is limited to the activity of isolated essential oils. Studies with different extracts, composition, and biological properties are still scarce. Aim of this study: The objective of this study was to evaluate the anti-inflammatory and anti-hyperalgesic activity of the hydroethanolic extract of L. canescens aerial parts. Materials and methods: The hydroethanolic extract L. canescens aerial parts (HELc) was analyzed using HPLC-DAD-EM. Male and female Swiss mice weighing 18–25 g were used in the in vivo assays. Acute toxicity was assessed (2000 mg/kg); anti-inflammatory activity through paw edema, mast cell degranulation and peritonitis, and anti-hyperalgesic activity through abdominal writhing assays induced by acetic acid and formalin sensitization, were evaluated using the doses of 3, 30 and 300 mg/kg. Results: The phytochemical characterization of HELc confirmed the presence of glycosylated iridoids (theveside, theviridoside), verbascosides and flavonoids. The HELc did not present toxicity in the evaluated dose. HELc reduced formation of paw edema, degranulation of peritoneal mast cells and infiltration of polymorphonuclear cells into the animals peritoneal cavity. In addition, HELc decreased the number of abdominal writhing induced by acetic acid and the time of paw licking in the evaluation of formalin sensitization. Conclusions: These results confirm the anti-inflammatory and anti-hyperalgesic effects of hydroethanolic extract of L. canescens, validating the use of this plant in folk medicine.

KW - Medicinal plants

KW - Preclinical assay

KW - Pain

KW - Inflammation

KW - Toxicity

UR - https://www.scopus.com/pages/publications/85111690922

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DO - 10.1016/j.jep.2021.114461

M3 - Article

C2 - 34333103

SN - 0378-8741

VL - 280

SP - 1

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JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

M1 - 114461

ER -

Lantana canescens (Kunth) inhibits inflammatory and hyperalgesic responses in murine models

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Keywords

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