Image-based ciliary beating frequency estimation for therapeutic assessment on defective mucociliary clearance diseases

Fan Zhang, Weidong Cai, Yang Song, Paul Young, Daniela Traini, Lucy Morgan, Hui Xin Ong, Lachlan Buddle, Dagan Feng

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

3 Citations (Scopus)

Abstract

Defective mucociliary clearance is inevitably seen in the chronic lung diseases and contributes substantially to their pathologies, and increasing the ciliary motility with external stimuli is considered to be efficient to relieve it. Ciliary Beating Frequency (CBF) is a regulated measurement to evaluate the drugs by comparing their effects on the motility of cilia. Therefore, CBF estimation is crucial for the therapeutic assessment on the defective mucociliary clearance diseases, and digital high speed imaging (DHSI) analysis provides an effective tool to measure CBF. However, the limitations of the current estimation approaches obstruct the accurate description of ciliary motility due to the introduced noise. Therefore, in this study, we propose a new image-based CBF estimation approach, which contains two main stages: a moving ciliated cell alignment is firstly conducted to remove the movement of ciliated cell, and then a threshold-based CBF estimation is designed to record the CBF by observing the beating cilia only. Different from the traditional approaches, the proposed scheme captures the most significant characteristics of the ciliary motility so that it can better describe the responses of the cilia to various drugs.

Original languageEnglish
Title of host publicationISBI 2014
Subtitle of host publicationProceedings of the 2014 IEEE 11th International Symposium on Biomedical Imaging
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages193-196
Number of pages4
ISBN (Electronic)9781467319614
ISBN (Print)9781467319591
DOIs
Publication statusPublished - 29 Jul 2014
Externally publishedYes
Event2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014 - Beijing, China
Duration: 29 Apr 20142 May 2014

Other

Other2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
CountryChina
CityBeijing
Period29/04/142/05/14

Keywords

  • Beating cilia
  • CBF estimation
  • Cell alignment
  • Mucociliary clearance

Fingerprint Dive into the research topics of 'Image-based ciliary beating frequency estimation for therapeutic assessment on defective mucociliary clearance diseases'. Together they form a unique fingerprint.

  • Cite this

    Zhang, F., Cai, W., Song, Y., Young, P., Traini, D., Morgan, L., ... Feng, D. (2014). Image-based ciliary beating frequency estimation for therapeutic assessment on defective mucociliary clearance diseases. In ISBI 2014: Proceedings of the 2014 IEEE 11th International Symposium on Biomedical Imaging (pp. 193-196). Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ISBI.2014.6867842