Immobilization of Pseudomonas sp. strain ADP: a stable inoculant for the bioremediation of atrazine

Scott Stelting, Richard G. Burns, Anwar Sunna, Gabriel Visnovsky, Craig R. Bunt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Storage and delivery of beneficial microorganisms are fundamental issues determining their value and effectiveness for a wide range of industrial and environmental purposes. One such application is the use of bacteria for the remediation of soil pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and persistent pesticides. Liquid cultures of a candidate for atrazine degradation in soil and water, Pseudomonas sp. strain ADP (3.67×10 9 colony-forming units, cfu/mL), when stored at 4 and 25°C, showed a 1 log reduction in cfu/mL occurs after approximately 4 and 2weeks, respectively. When immobilized onto natural zeolite from two sources (a New Zealand and an Australian quarry) and stored in open containers exposed to the atmosphere, survival at 25°C was poor. However, when the cells were immobilized onto the Australian zeolite with xanthan gum and stored in closed containers, survival at 25°C was superior to control cells stored at 4°C. The initial growth medium, zeolite substratum and immobilization matrix excipients all appear to play an important role in the stabilization of Pseudomonas sp. strain ADP. The bacteria immobilized onto Australian zeolite with xanthan remained viable within 1 log unit of initial cfu/g loading and retained their ability to degrade atrazine (as measured by zone clearance on atrazine containing plates) for at least 10weeks at 25°C.

LanguageEnglish
Pages90-93
Number of pages4
JournalApplied Clay Science
Volume64
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Atrazine
Zeolites
Bioremediation
atrazine
bioremediation
zeolite
Adenosine Diphosphate
immobilization
Containers
Bacteria
Soil Pollutants
bacterium
Quarries
Excipients
Polychlorinated Biphenyls
Polycyclic Aromatic Hydrocarbons
Remediation
Pesticides
Microorganisms
quarry

Cite this

Stelting, Scott ; Burns, Richard G. ; Sunna, Anwar ; Visnovsky, Gabriel ; Bunt, Craig R. / Immobilization of Pseudomonas sp. strain ADP : a stable inoculant for the bioremediation of atrazine. In: Applied Clay Science. 2012 ; Vol. 64. pp. 90-93.
@article{a3d43b2e289d4fdd859265556841e074,
title = "Immobilization of Pseudomonas sp. strain ADP: a stable inoculant for the bioremediation of atrazine",
abstract = "Storage and delivery of beneficial microorganisms are fundamental issues determining their value and effectiveness for a wide range of industrial and environmental purposes. One such application is the use of bacteria for the remediation of soil pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and persistent pesticides. Liquid cultures of a candidate for atrazine degradation in soil and water, Pseudomonas sp. strain ADP (3.67×10 9 colony-forming units, cfu/mL), when stored at 4 and 25°C, showed a 1 log reduction in cfu/mL occurs after approximately 4 and 2weeks, respectively. When immobilized onto natural zeolite from two sources (a New Zealand and an Australian quarry) and stored in open containers exposed to the atmosphere, survival at 25°C was poor. However, when the cells were immobilized onto the Australian zeolite with xanthan gum and stored in closed containers, survival at 25°C was superior to control cells stored at 4°C. The initial growth medium, zeolite substratum and immobilization matrix excipients all appear to play an important role in the stabilization of Pseudomonas sp. strain ADP. The bacteria immobilized onto Australian zeolite with xanthan remained viable within 1 log unit of initial cfu/g loading and retained their ability to degrade atrazine (as measured by zone clearance on atrazine containing plates) for at least 10weeks at 25°C.",
author = "Scott Stelting and Burns, {Richard G.} and Anwar Sunna and Gabriel Visnovsky and Bunt, {Craig R.}",
year = "2012",
month = "8",
doi = "10.1016/j.clay.2011.12.006",
language = "English",
volume = "64",
pages = "90--93",
journal = "Applied Clay Science",
issn = "0169-1317",
publisher = "Elsevier",

}

Immobilization of Pseudomonas sp. strain ADP : a stable inoculant for the bioremediation of atrazine. / Stelting, Scott; Burns, Richard G.; Sunna, Anwar; Visnovsky, Gabriel; Bunt, Craig R.

In: Applied Clay Science, Vol. 64, 08.2012, p. 90-93.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Immobilization of Pseudomonas sp. strain ADP

T2 - Applied Clay Science

AU - Stelting, Scott

AU - Burns, Richard G.

AU - Sunna, Anwar

AU - Visnovsky, Gabriel

AU - Bunt, Craig R.

PY - 2012/8

Y1 - 2012/8

N2 - Storage and delivery of beneficial microorganisms are fundamental issues determining their value and effectiveness for a wide range of industrial and environmental purposes. One such application is the use of bacteria for the remediation of soil pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and persistent pesticides. Liquid cultures of a candidate for atrazine degradation in soil and water, Pseudomonas sp. strain ADP (3.67×10 9 colony-forming units, cfu/mL), when stored at 4 and 25°C, showed a 1 log reduction in cfu/mL occurs after approximately 4 and 2weeks, respectively. When immobilized onto natural zeolite from two sources (a New Zealand and an Australian quarry) and stored in open containers exposed to the atmosphere, survival at 25°C was poor. However, when the cells were immobilized onto the Australian zeolite with xanthan gum and stored in closed containers, survival at 25°C was superior to control cells stored at 4°C. The initial growth medium, zeolite substratum and immobilization matrix excipients all appear to play an important role in the stabilization of Pseudomonas sp. strain ADP. The bacteria immobilized onto Australian zeolite with xanthan remained viable within 1 log unit of initial cfu/g loading and retained their ability to degrade atrazine (as measured by zone clearance on atrazine containing plates) for at least 10weeks at 25°C.

AB - Storage and delivery of beneficial microorganisms are fundamental issues determining their value and effectiveness for a wide range of industrial and environmental purposes. One such application is the use of bacteria for the remediation of soil pollutants such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and persistent pesticides. Liquid cultures of a candidate for atrazine degradation in soil and water, Pseudomonas sp. strain ADP (3.67×10 9 colony-forming units, cfu/mL), when stored at 4 and 25°C, showed a 1 log reduction in cfu/mL occurs after approximately 4 and 2weeks, respectively. When immobilized onto natural zeolite from two sources (a New Zealand and an Australian quarry) and stored in open containers exposed to the atmosphere, survival at 25°C was poor. However, when the cells were immobilized onto the Australian zeolite with xanthan gum and stored in closed containers, survival at 25°C was superior to control cells stored at 4°C. The initial growth medium, zeolite substratum and immobilization matrix excipients all appear to play an important role in the stabilization of Pseudomonas sp. strain ADP. The bacteria immobilized onto Australian zeolite with xanthan remained viable within 1 log unit of initial cfu/g loading and retained their ability to degrade atrazine (as measured by zone clearance on atrazine containing plates) for at least 10weeks at 25°C.

UR - http://www.scopus.com/inward/record.url?scp=84865443665&partnerID=8YFLogxK

U2 - 10.1016/j.clay.2011.12.006

DO - 10.1016/j.clay.2011.12.006

M3 - Article

VL - 64

SP - 90

EP - 93

JO - Applied Clay Science

JF - Applied Clay Science

SN - 0169-1317

ER -