Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

D. Tewari; S. V. Nair; M. C. De Ungria; G. L. Lawrence; M. Hayden; D. N. Love; H. J. Field; J. M. Whalley

doi:10.1007/BF01309967

Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

D. Tewari^*, S. V. Nair, M. C. De Ungria, G. L. Lawrence, M. Hayden, D. N. Love, H. J. Field, J. M. Whalley

^*Corresponding author for this work

Faculty of Science and Engineering

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.

Original language	English
Pages (from-to)	789-797
Number of pages	9
Journal	Archives of Virology
Volume	140
Issue number	4
DOIs	https://doi.org/10.1007/BF01309967
Publication status	Published - Apr 1995

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title = "Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model",

abstract = "The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.",

author = "D. Tewari and Nair, {S. V.} and {De Ungria}, {M. C.} and Lawrence, {G. L.} and M. Hayden and Love, {D. N.} and Field, {H. J.} and Whalley, {J. M.}",

year = "1995",

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doi = "10.1007/BF01309967",

language = "English",

volume = "140",

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T1 - Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

AU - Tewari, D.

AU - Nair, S. V.

AU - De Ungria, M. C.

AU - Lawrence, G. L.

AU - Hayden, M.

AU - Love, D. N.

AU - Field, H. J.

AU - Whalley, J. M.

PY - 1995/4

Y1 - 1995/4

N2 - The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.

AB - The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.

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Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

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