Immunocytochemical identification of CD5 positive peripheral blood lymphocytes in four marsuipial species

L. J. Young, E. M. Deane*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Immunocytochemical analysis of peripheral blood mononuclear cells was undertaken using a streptavidin biotin-horseradish peroxidase method to detect CD5 positive lymphocytes from the blood of several marsupial species. A monoclonal antibody raised to a conserved peptide sequence of the human CD5 antigen positively labelled lymphocytes in freshly isolated peripheral blood mononuclear cells of the tammar wallaby (Macropus eugenii), the long-footed potoroo (Potorous longipes), the long-nosed potoroo (Potorous tridactylus) and the rufous hare-wallaby (Lagorchestes hirsutus). A polyclonal anti-CD3 antibody also positively labelled circulating lymphocytes from the tammar wallaby. Whereas previous studies using flow cytometry reported labelling of T cells in koala lymphocyte preparations using a polyclonal anti-CD3 antibody, there have been no other reports of marsupial blood immunophenotyping. The current study extends the known applications of monoclonal anti-CD5 and polyclonal anti-CD3 antibodies to blood lymphocytes of small wallaby species using an immunocytochemical slide technique that is simple, can be processed within a day and requires no dedicated large equipment.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalComparative Clinical Pathology
Volume12
Issue number1
DOIs
Publication statusPublished - May 2003

Keywords

  • CD5
  • Immunohistochemistry
  • Lymphocyte
  • Marsupial
  • Potoroo
  • Wallaby

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