Peptides derived from the bee-venom melittin were fitted with the haptenic group dinitrocarboxyphenyl (Dncp) and tested in out-bred guinea pigs for immunogenicity by measuring the IgG anti-Dncp antibody response by ELISA. Dncp-conjugates comprising virtually the entire melittin proved to be strong immunogens producing antibody responses comparable to those of proteins. Weak responses were obtained with considerably shortened sequences. Conjugates with N-terminal Dncp gave markedly reduced antibody responses compared to peptides with C-terminal Dncp. An N-terminal biotinyl substituent abolished the immune response whereas N-terminal lauryl and caprylyl had little effect. Insertion of L-proline into a hexadecapeptide conjugate abolishing the possibility of helix formation gave an immunogen to which individual animals clearly responded on a low level. Oligomerisation, but not the cytolytic activity of melittin peptides, may contribute to the immunogenicities observed.
|Number of pages||2|
|Publication status||Published - 1994|
|Event||23rd European peptide symposium - Braga, Portugal|
Duration: 4 Sep 1994 → 10 Sep 1994
|Conference||23rd European peptide symposium|
|Period||4/09/94 → 10/09/94|
Curcio-Vonlanthen, V., Zhao, Z., Rolli, H. P., & Schneider, C. H. (1994). Immunogenicity changes in melittin conjugates by structural modifications. 843-844. Paper presented at 23rd European peptide symposium, Braga, Portugal.