Immunogenicity of dinitrocarboxyphenylated melittin

the influence of C-terminal chain shortening, N-terminal substitution and prolin insertion at positions 5 and 10

Zhenjun Zhao, Hanspeter Rolli, Conrad H. Schneider

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Peptides derived from the bee-venom melittin were fitted with the haptenic group dinitrocarboxyphenyl(Dncp) and tested in out-bred guinea pigs for immunogenicity by measuring the IgG anti-Dncp antibody response by ELISA. Dncp-conjugates comprising virtually the entire melittin proved to be strong immunogens producing antibody responses comparable to those of proteins. Weak responses were obtained with considerably shortened seqences. Conjugates with N-terminal Dncp gave markedly reduced antibody responses compared to peptides with C-terminal Dncp. An N-terminal biotinyl substituent abolished the immune response whereas N-terminal lauryl and caprylyl had little effect. Insertion of L-proline into a hexadecapeptide conjugate abolishing the possibility of helix formation gave an immunogen to which individual animals clearly responded on a low level. Oligomerisation, but not the cytolytic activity of melittin peptides, may contribute to the immunogenicities observed.
Original languageEnglish
Pages (from-to)140-148
Number of pages9
JournalJournal of Peptide Science
Volume1
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • melittin immunogenicity
  • T-cell epitopic secondary structure
  • melittin toxicity
  • substituent effects

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